Proteomics

Dataset Information

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Enrichment strategy for searching missing protein


ABSTRACT: Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modification (PTM), and nucleic acid associated.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Blood Serum

SUBMITTER: Na Su  

LAB HEAD: Ping Xu

PROVIDER: PXD002255 | Pride | 2015-07-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ES_XP_LMW_DS_117.dat-pride.pride.mgf.gz Mgf
ES_XP_LMW_DS_117.dat-pride.pride.mztab.gz Mztab
ES_XP_LMW_DS_117.dat-pride.xml.gz Xml
ES_XP_LMW_DS_117.raw Raw
ES_XP_LMW_DS_118.dat-pride.pride.mgf.gz Mgf
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Publications

Special Enrichment Strategies Greatly Increase the Efficiency of Missing Proteins Identification from Regular Proteome Samples.

Su Na N   Zhang Chengpu C   Zhang Yao Y   Wang Zhiqiang Z   Fan Fengxu F   Zhao Mingzhi M   Wu Feilin F   Gao Yuan Y   Li Yanchang Y   Chen Lingsheng L   Tian Miaomiao M   Zhang Tao T   Wen Bo B   Sensang Na N   Xiong Zhi Z   Wu Songfeng S   Liu Siqi S   Yang Pengyuan P   Zhen Bei B   Zhu Yunping Y   He Fuchu F   Xu Ping P  

Journal of proteome research 20150715 9


As part of the Chromosome-Centric Human Proteome Project (C-HPP) mission, laboratories all over the world have tried to map the entire missing proteins (MPs) since 2012. On the basis of the first and second Chinese Chromosome Proteome Database (CCPD 1.0 and 2.0) studies, we developed systematic enrichment strategies to identify MPs that fell into four classes: (1) low molecular weight (LMW) proteins, (2) membrane proteins, (3) proteins that contained various post-translational modifications (PTM  ...[more]

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