Proteomics

Dataset Information

0

Kv7.2 LC-MSMS - Updating in vivo and in vitro phosphorylation and methylation sites of voltage-gated Kv7

ABSTRACT: Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of Kv7 channels is regulated by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signalling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2), Ca2+/Calmodulin and phosphorylation. Recent studies have reported that PIP2 sensitivity of Kv7.2 channels is affected by two PTMs, phosphorylation and methylation, harboured in the PIP2 binding domains. Here this project aimed to update phosphorylation and methylation sites on Kv7.2 from heterologous cells, the rat brain and GST-fusion Kv7.2 N- and C-terminal ends proteins.

INSTRUMENT(S): Bruker Daltonics micrOTOF series, Bruker Daltonics amaZon series

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Brain, Hek-293 Cell

SUBMITTER: Jae-Won Yang  

LAB HEAD: Jae-Won Yang

PROVIDER: PXD005567 | Pride | 2017-08-25

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
10_GST-Kv72N+DNAPK_methyl.pride.mgf.gz Mgf
10_GST-Kv72N+DNAPK_methyl_F006204.pride.mztab.gz Mztab
10_GST-Kv72N+DNAPK_phos.pride.mgf.gz Mgf
10_GST-Kv72N+DNAPK_phos_F006203.pride.mztab.gz Mztab
10_GST-Kv72NDNAPK_methyl.baf Other
Items per page:
1 - 5 of 141
altmetric image

Publications

Updating In Vivo and In Vitro Phosphorylation and Methylation Sites of Voltage-Gated Kv7.2 Potassium Channels.

Erdem Fatma Asli FA   Salzer Isabella I   Heo Seok S   Chen Wei-Qiang WQ   Jung Gangsoo G   Lubec Gert G   Boehm Stefan S   Yang Jae-Won JW  

Proteomics 20171001 19

Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of these channels is tightly controlled by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signaling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP<sub>2</sub> ), Ca<sup>2+</sup> /calmodulin, and phosphorylation. Recent studies found that the PIP<sub>2</sub> sensitivity of Kv7.2 channels is affected by two posttranslational modifications, phosphorylation a  ...[more]

Similar Datasets

Proteomics Proteomic Analysis of Protein Methylation in the Yeast Saccharomyces cerevisiae
2020-01-27 | PXD000606 | Pride
Proteomics METTL13 modulates mRNA translation through methylation of eEF1A at Lys55 and the N-terminus, part 2
2018-07-04 | PXD009914 | Pride
Proteomics MS_HistoneDB, a manually curated resource for proteomic analysis of human and mouse histones
2017-01-11 | PXD005489 | Pride
Proteomics Lysine benzoylation - Here we report the identification and characterization of a histone mark, lysine benzoylation (Kbz). Our study identifies 22 Kbz sites on histones from HepG2 and RAW cells. This type of histone mark can be stimulated by sodium benzoate (SB), a FDA-approved drug and a widely used chemical food preservative, via generation of benzoyl CoA.
2018-07-11 | PXD010332 | Pride
Proteomics,Multiomics Chromatin proteomics reveals novel combinatorial histone modification signatures that mark distinct subpopulations of macrophage enhancers
2017-10-09 | PXD007582 | Pride
Proteomics Phosphorylation of the FACT histone chaperone subunit SPT16 modulates Arabidopsis development and affects chromatin at RNA polymerase II transcriptional start sites
2022-06-09 | PXD028534 | Pride
Proteomics Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans
2023-07-03 | PXD042540 | Pride
Proteomics Characterization of Arginine Methylation in Cancer Cell using LC-MS/MS
2024-01-11 | PXD039441 | Pride
Proteomics Characterization of METTL9-mediated methylation of DNAJB12
2020-11-19 | PXD020010 | Pride
Proteomics Tracking DOT1L Methyltransferase Activity by Stable Isotope Labelling Using a Selective Synthetic Co-Factor
2024-06-28 | PXD052790 | Pride