Dataset Information

Large scale site-specific mapping of the Arabidopsis ubiquitinome

ABSTRACT: Post-translational modification of amino acids changes the properties of a protein and increases the functional diversity of the proteome. For example, protein ubiquitination influences almost all cellular processes and can remodel the proteome in a matter of minutes. However, mostly due to technical limitations, global profiling of the plant ubiquitinome and proteome-wide identification of specific ubiquitinated residues remains challenging. Here, we made use of the COFRADIC technology to map ubiquitination sites in Arabidopsis thaliana. Across two biological replicates, we identified a total of 3,009 ubiquitination sites in 1,607 proteins. Our data increases the number of reported ubiquitin conjugation sites in Arabidopsis by a factor of 10. It contains well-known ubiquitination targets, including cell cycle proteins and key transcriptional regulators of phytohormone signaling as well as yet unreported targets, such as the Glutaredoxin S17 for which we provide here evidence of proteasomal degradation Finally, we could detect N-terminal ubiquitin conjugation on 19 proteins, a less commonly described type of protein modification.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Culture

SUBMITTER: Alan Walton

LAB HEAD: Kris Gevaert

PROVIDER: PXD002297 | Pride | 2016-08-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	E07356_2p_25_Alan_ubicofradic_fr21.raw	Raw
	E07357_1p_25_Alan_ubicofradic_fr22.raw	Raw
	E07358_2p_25_Alan_ubicofradic_fr23.raw	Raw
	E07359_1p_25_Alan_ubicofradic_fr24.raw	Raw
	E07360_2p_25_Alan_ubicofradic_fr25.raw	Raw

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Publications

It's Time for Some "Site"-Seeing: Novel Tools to Monitor the Ubiquitin Landscape in Arabidopsis thaliana.

Walton Alan A Stes Elisabeth E Cybulski Nicolas N Van Bel Michiel M Iñigo Sabrina S Durand Astrid Nagels AN Timmerman Evy E Heyman Jefri J Pauwels Laurens L De Veylder Lieven L Goossens Alain A De Smet Ive I Coppens Frederik F Goormachtig Sofie S Gevaert Kris K

The Plant cell 20160107 1

Ubiquitination, the covalent binding of the small protein modifier ubiquitin to a target protein, is an important and frequently studied posttranslational protein modification. Multiple reports provide useful insights into the plant ubiquitinome, but mostly at the protein level without comprehensive site identification. Here, we implemented ubiquitin combined fractional diagonal chromatography (COFRADIC) for proteome-wide ubiquitination site mapping on Arabidopsis thaliana cell cultures. We iden ...[more]

PMID: 26744219

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