Dataset Information

The dynamic regulatory genome of Capsaspora owczarzaki and the origin of animal multicellularity

ABSTRACT: Recent evidence that the unicellular ancestor of animals had a complex repertoire of genes linked to multicellular processes suggests that changes in the regulatory genome, rather than gene innovation, were key to the origin of animals. Here, we carry out multiple functional genomic assays in Capsaspora owczarzaki, the unicellular relative of animals with the largest known gene repertoire for transcriptional regulation. We show that changing chromatin states, differential lincRNA expression and dynamic cis-regulatory sites are associated with life cycle transitions in Capsaspora. Moreover, we demonstrate conservation of animal developmental transcription factor networks and extensive network interconnection in this premetazoan organism. In contrast, however, Capsaspora lacks animal promoter types and its regulatory sites are small, proximal and lack signatures of animal enhancers. Overall, our results indicate that the emergence of animal multicellularity was linked to a major shift in genome regulatory complexity, most notably the appearance of distal enhancer regulation.

OTHER RELATED OMICS DATASETS IN: PRJNA290432

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Capsaspora Owczarzaki

SUBMITTER: Cristina Chiva

LAB HEAD: Iñaki Ruiz

PROVIDER: PXD002342 | Pride | 2018-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	140129_S_ARNAU_01_01_CoHist_Capsaspora_derivat_1st_elution_5ug.msf	Msf
	140129_S_ARNAU_01_02_CoHist_Capsaspora_derivat_2nd_elution_5ug2.msf	Msf
	140131_S_ARNAU_01_03_CoHist_Capsaspora_derivat_velos_5ug_1st_elution.raw	Raw
	140131_S_ARNAU_01_04_CoHist_Capsaspora_derivat_velos_5ug_2nd_elution.raw	Raw
	140211_S_ARNAU_01_04_CoHist_Capsaspora_derivat_velos_HCD_5ug_1st_2nd_elution.msf	Msf

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Publications

The Dynamic Regulatory Genome of Capsaspora and the Origin of Animal Multicellularity.

Sebé-Pedrós Arnau A Ballaré Cecilia C Parra-Acero Helena H Chiva Cristina C Tena Juan J JJ Sabidó Eduard E Gómez-Skarmeta José Luis JL Di Croce Luciano L Ruiz-Trillo Iñaki I

Cell 20160421 5

The unicellular ancestor of animals had a complex repertoire of genes linked to multicellular processes. This suggests that changes in the regulatory genome, rather than in gene innovation, were key to the origin of animals. Here, we carry out multiple functional genomic assays in Capsaspora owczarzaki, the unicellular relative of animals with the largest known gene repertoire for transcriptional regulation. We show that changing chromatin states, differential lincRNA expression, and dynamic cis ...[more]

PMID: 27114036

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