Dataset Information

The Lipid Raft Proteome of Borrelia burgdorferi.

ABSTRACT: Eukaryotic lipid rafts are membrane microdomains rich in cholesterol that contain a selective set of proteins, and have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol onto its membrane, and form cholesterol glycolipid domains that possess all the hallmarks of eukaryotic lipid rafts. In this study, we isolated lipid rafts from cultured B. burgdorferi as a detergent resistant membrane (DRM) fraction on density gradients, and characterized those molecules that partitioned exclusively or are highly enriched in these domains. Cholesterol glycolipids, the previously known raft-associated lipoproteins OspA and OpsB, and cholera toxin partitioned into the lipid rafts fraction indicating compatibility with components of the DRM. The proteome of lipid rafts was analyzed by a combination of LC-MS/MS or MudPIT. Identified proteins were analyzed in silico for parameters that included localization, isoelectric point, molecular mass and biological function. The proteome provided a consistent pattern of lipoproteins, proteases and their substrates, sensing molecules, and prokaryotic homologs of eukaryotic lipid rafts. This study provides the first analysis of a prokaryotic lipid raft and has relevance for the biology of Borrelia, other pathogenic bacteria, as well as for the evolution of these structures.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Borrelia Burgdorferi B31

TISSUE(S): Prokaryotic Cell

DISEASE(S): Lyme Disease

SUBMITTER: Alvaro Toledo

LAB HEAD: Jorge Benach

PROVIDER: PXD002365 | Pride | 2015-09-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	tk101224_Alvaro_1_01.RAW	Raw
	tk101224_Alvaro_1_01.mzid.gz	Mzid
	tk101224_Alvaro_1_01.mzid_tk101224_Alvaro_1_01.MGF	Mzid
	tk101224_Alvaro_1_01.mzid_tk101224_Alvaro_1_01.pride.mgf.gz	Mzid
	tk101224_Alvaro_1_01.pride.mztab.gz	Mztab

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The lipid raft proteome of Borrelia burgdorferi.

Toledo Alvaro A Pérez Alberto A Coleman James L JL Benach Jorge L JL

Proteomics 20150928 21

Eukaryotic lipid rafts are membrane microdomains that have significant amounts of cholesterol and a selective set of proteins that have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol onto its membrane, and form cholesterol glycolipid domains that possess all the hallmarks of eukaryotic lipid rafts. In this study, we isolated lipid rafts from cultured B. burgdorfe ...[more]

PMID: 26256460

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