Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)
TISSUE(S): Spleen, T Cell, Epithelial Cell, Cell Culture
SUBMITTER: Cerina Chhuon
LAB HEAD: Ida Chiara Guerrera
PROVIDER: PXD016476 | Pride | 2020-08-13
REPOSITORIES: Pride
Items per page: 5 1 - 5 of 69 |
Chhuon Cerina C Zhang Shao-Yu SY Jung Vincent V Lewandowski Daniel D Lipecka Joanna J Pawlak André A Sahali Dil D Ollero Mario M Guerrera Ida Chiara IC
Journal of lipid research 20200807 11
The analysis of T cell lipid raft proteome is challenging due to the highly dynamic nature of rafts and the hydrophobic character of raft-resident proteins. We explored an innovative strategy for bottom-up lipid raftomics based on suspension-trapping (S-Trap) sample preparation. Mouse T cells were prepared from splenocytes by negative immunoselection, and rafts were isolated by a detergent-free method and OptiPrep gradient ultracentrifugation. Microdomains enriched in flotillin-1, LAT, and chole ...[more]