Proteomics

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The response of Chlamydomonas reinhardtii to nitrogen deprivation: a systems biology analysis


ABSTRACT: Drastic alteration in macronutrients causes large changes in gene expression in the photosynthetic unicellular alga Chlamydomonas reinhardtii. Preliminary data suggested that cells follow a biphasic response to this change hinging on the initiation of lipid accumulation, and we hypothesized that drastic repatterning of metabolism also followed this biphasic modality. To test this hypothesis, transcriptomic, proteomic, and metabolite changes that occur under nitrogen (N) deprivation were analyzed. Eight sampling times were selected covering the progressive slowing of growth and induction of oil synthesis between 4 and 6 h after N deprivation. Results of the combined, systems-level investigation indicated that C. reinhardtii cells sense and respond on a large scale within 30 min to a switch to N-deprived conditions turning on a largely gluconeogenic metabolic state, which then transitions to a glycolytic stage between 4 and 6 h after N depletion. This nitrogen-sensing system is transduced to carbon- and nitrogen-responsive pathways, leading to down-regulation of carbon assimilation and chlorophyll biosynthesis, and an increase in nitrogen metabolism and lipid biosynthesis. For example, the expression of nearly all the enzymes for assimilating nitrogen from ammonium, nitrate, nitrite, urea, formamide/acetamide, purines, pyrimidines, polyamines, amino acids and proteins increased significantly. Although arginine biosynthesis enzymes were also rapidly up-regulated, arginine pool size changes and isotopic labeling results indicated no increased flux through this pathway.

OTHER RELATED OMICS DATASETS IN: MTBLS208MTBLS90

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Chlamydomonas Reinhardtii

SUBMITTER: Evan McConnell  

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD002377 | Pride | 2015-06-17

REPOSITORIES: Pride

Dataset's files

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Action DRS
F008739_S2_1.mzid.gz Mzid
F008739_S2_1.pride.mztab.gz Mztab
F008740_S2_2.mzid.gz Mzid
F008740_S2_2.pride.mztab.gz Mztab
F008741_S3_1.mzid.gz Mzid
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The response of Chlamydomonas reinhardtii to nitrogen deprivation: a systems biology analysis.

Park Jeong-Jin JJ   Wang Hongxia H   Gargouri Mahmoud M   Deshpande Rahul R RR   Skepper Jeremy N JN   Holguin F Omar FO   Juergens Matthew T MT   Shachar-Hill Yair Y   Hicks Leslie M LM   Gang David R DR  

The Plant journal : for cell and molecular biology 20150201 4


Drastic alteration in macronutrients causes large changes in gene expression in the photosynthetic unicellular alga Chlamydomonas reinhardtii. Preliminary data suggested that cells follow a biphasic response to this change hinging on the initiation of lipid accumulation, and we hypothesized that drastic repatterning of metabolism also followed this biphasic modality. To test this hypothesis, transcriptomic, proteomic, and metabolite changes that occur under nitrogen (N) deprivation were analyzed  ...[more]

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