Proteomics

Dataset Information

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Quantitative cross-linking analysis of chemoresistant HeLa cells

ABSTRACT: Chemoresistance is a common mode of therapy failure for many cancers. Tumors develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions contributing to the development of new phenotypes. The ability to understand how protein interaction networks adapt to yield new function or alter phenotype is limited by the inability to determine structural and protein interaction changes on a proteomic scale. In this project we combine SILAC with PIR cross-linking technology to generate a quantitative protein interaction map of drug senstive and drug resistant HeLa cells.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Juan Chavez  

LAB HEAD: James E. Bruce

PROVIDER: PXD002378 | Pride | 2015-09-15

REPOSITORIES: pride

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Dataset's files

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Action DRS
021612_HeLa_SILAC_BDP_7_8_100mM_2hr.raw Raw
021612_HeLa_SILAC_BDP_7_8_150mM_2hr.raw Raw
021612_HeLa_SILAC_BDP_7_8_200mM_2hr.raw Raw
021612_HeLa_SILAC_BDP_7_8_50mM_2hr.raw Raw
021712_HeLa_SILAC_BDP_7_8_100mM_4hr.raw Raw
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Publications

Quantitative interactome analysis reveals a chemoresistant edgotype.

Chavez Juan D JD   Schweppe Devin K DK   Eng Jimmy K JK   Zheng Chunxiang C   Taipale Alex A   Zhang Yiyi Y   Takara Kohji K   Bruce James E JE  

Nature communications 20150803

Chemoresistance is a common mode of therapy failure for many cancers. Tumours develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions contributing to the development of new phenotypes. The ability to understand how protein interaction networks adapt to yield new function or alter phenotype is limited by the inability to determine structu  ...[more]

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