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Quantitative cross-linking analysis of chemoresistant HeLa cells

ABSTRACT: Chemoresistance is a common mode of therapy failure for many cancers. Tumors develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions contributing to the development of new phenotypes. The ability to understand how protein interaction networks adapt to yield new function or alter phenotype is limited by the inability to determine structural and protein interaction changes on a proteomic scale. In this project we combine SILAC with PIR cross-linking technology to generate a quantitative protein interaction map of drug senstive and drug resistant HeLa cells.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Juan Chavez

LAB HEAD: James E. Bruce

PROVIDER: PXD002378 | Pride | 2015-09-15

REPOSITORIES: Pride

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Quantitative interactome analysis reveals a chemoresistant edgotype.

Chavez Juan D JD Schweppe Devin K DK Eng Jimmy K JK Zheng Chunxiang C Taipale Alex A Zhang Yiyi Y Takara Kohji K Bruce James E JE

Nature communications 20150803

Chemoresistance is a common mode of therapy failure for many cancers. Tumours develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions contributing to the development of new phenotypes. The ability to understand how protein interaction networks adapt to yield new function or alter phenotype is limited by the inability to determine structu ...[more]

PMID: 26235782

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