Proteomics

Dataset Information

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Chemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue.

ABSTRACT: While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically non-existent. Many protein properties and their involvement in disease pathways are not replicated in cell culture and some complexes are not amenable to purification. Thus, the lack of tissue-level structural information limits molecular-level insight on diseases such as heart failure. Here we demonstrate the application of XL-MS to identify protein structural features and interactions in tissue samples, providing the first systems structural biology insight on protein complexes as they exist in the mouse heart.

INSTRUMENT(S): LTQ FT, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Juan Chavez  

LAB HEAD: James E. Bruce

PROVIDER: PXD007673 | Pride | 2017-11-30

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
021617_heart_mito_postXL_F10_d8BDP_2hr1.pep.xml.react2.xls Pepxml
021617_heart_mito_postXL_F10_d8BDP_2hr1.raw Raw
021617_heart_mito_postXL_F10_d8BDP_2hr1.raw.txt Raw
021617_heart_mito_postXL_F11_14_d8BDP_2hr1.pep.xml.react2.xls Pepxml
021617_heart_mito_postXL_F11_14_d8BDP_2hr1.raw Raw
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Publications

Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue.

Chavez Juan D JD   Lee Chi Fung CF   Caudal Arianne A   Keller Andrew A   Tian Rong R   Bruce James E JE  

Cell systems 20171129 1

While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically nonexistent. Here, we demonstrate the application of crosslinking mass spectrometry to identify protein structural features and interactions in tissue samples, providing systems structural biology insight into protein complexes as they exist  ...[more]

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