Dataset Information

EV71 proteomics - Analysis of EV71 infection progression using triple-SILAC-based proteomics approach

ABSTRACT: Enterovirus 71 (EV71), a member of Picornaviridae, causes severe neurological and systemic illness in children. To better understand the virus-host cell interactions, we performed a triple-SILAC-based quantitative proteomics study monitoring host cell proteome changes after EV71 infection. Based on the quantitative data for more than 4100 proteins, ~17% of the proteins were found as significantly changed (p<0.01) at either 8 or 20 hours post infection (h.p.i.). Five biological processes and seven protein classes showed significant differences. Functional screening of 9 regulated proteins discovered the regulatory role of CHCH2, a mitochondrial protein known as a transcriptional activator for cytochrome c oxidase (COX), in EV71 replication. Further studies showed that CHCH2 served as a negative regulator of innate immune responses.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Haoyu Li

LAB HEAD: Lin Guo

PROVIDER: PXD002483 | Pride | 2015-08-31

REPOSITORIES: Pride

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Publications

The predictive value of cumulative lactate dehydrogenase release within the first 72 h of acute myocardial infarction in patients treated with primary angioplasty.

Elsman Peter P Zijlstra Felix F Miedema Kor K Hoorntje Jan C A JC Dikkeschei Lambert D LD Slingerland Robbert J RJ Reiffers Stoffer S de Boer Menko-Jan MJ Suryapranata Harry H

Annals of clinical biochemistry 20040301 Pt 2

<h4>Background</h4>In patients with acute myocardial infarction, estimation of infarct size by cumulative lactate dehydrogenase release at 72 h (LDHQ(72)) is a simple and widely used method. Our objective was to study the value of estimating infarct size, by the cumulative release of LDH over 72, 60, 48 and 36 h in predicting left ventricular ejection fraction (LV(ef)) and cardiac death at 1 year.<h4>Methods</h4>In the Zwolle Infarction Study infarct size estimated as LDHQ was calculated in 1224 ...[more]

PMID: 15025806

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