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RBDmap - The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease

ABSTRACT: RBDmap protein regions contacting RNA were crosslinked with UV and captured with oligodT beads

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Cardiac Muscle Cell

SUBMITTER: Sophia Foehr

LAB HEAD: Jeroen Krijgsveld

PROVIDER: PXD002543 | Pride | 2016-08-17

REPOSITORIES: Pride

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			Action	DRS
	140124_CF_YL_RBDmap_rep1_f1_40p.raw	Raw
	140124_CF_YL_RBDmap_rep1_f2_40p.raw	Raw
	140124_CF_YL_RBDmap_rep1_f3_40p.raw	Raw
	140124_CF_YL_RBDmap_rep1_f4_40p.raw	Raw
	140124_CF_YL_RBDmap_rep1_f5_40p.raw	Raw

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Publications

The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease.

Liao Yalin Y Castello Alfredo A Fischer Bernd B Leicht Stefan S Föehr Sophia S Frese Christian K CK Ragan Chikako C Kurscheid Sebastian S Pagler Eloisa E Yang Hao H Krijgsveld Jeroen J Hentze Matthias W MW Preiss Thomas T

Cell reports 20160721 5

RNA functions through the dynamic formation of complexes with RNA-binding proteins (RBPs) in all clades of life. We determined the RBP repertoire of beating cardiomyocytic HL-1 cells by jointly employing two in vivo proteomic methods, mRNA interactome capture and RBDmap. Together, these yielded 1,148 RBPs, 391 of which are shared with all other available mammalian RBP repertoires, while 393 are thus far unique to cardiomyocytes. RBDmap further identified 568 regions of RNA contact within 368 RBP ...[more]

PMID: 27452465

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HL-1 mRNA interactome - The Cardiomyocyte RNA-Binding Proteome: Links to Intermediary Metabolism and Heart Disease

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2016-08-17 | PXD002541 | Pride

Differential Protein Occupancy Profiling of the mRNA Transcriptome

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