Proteomics

Dataset Information

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Fusarium graminearum in vitro cellular proteome


ABSTRACT: The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum. This dataset includes the cellular control sample that was analysed with shotgun mass-spec proteomics followed SearchGUI and Peptide shaker searches.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Gibberella Zeae (strain Ph-1 / Atcc Mya-4620 / Fgsc 9075 / Nrrl 31084) (wheat Head Blight Fungus) (fusarium Graminearum)

TISSUE(S): Hypha, Fungal Cell

SUBMITTER: Rohan Lowe  

LAB HEAD: Marilyn Anderson

PROVIDER: PXD002840 | Pride | 2015-10-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BSA_OM.raw Raw
BSA_OM_1.mgf Mgf
BSA_OM_1.pride.mgf.gz Mgf
BSA_OM_2.mgf Mgf
BSA_OM_2.pride.mgf.gz Mgf
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Publications

Extracellular peptidases of the cereal pathogen Fusarium graminearum.

Lowe Rohan G T RG   McCorkelle Owen O   Bleackley Mark M   Collins Christine C   Faou Pierre P   Mathivanan Suresh S   Anderson Marilyn M  

Frontiers in plant science 20151106


The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality, and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of th  ...[more]

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