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Tomato quantitative proteome - Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3

ABSTRACT: Identification of protease targets is essential to understand the molecular mechanism by which a protease regulates the biological processes. In this study, we applied an iTRAQ-based quantitative proteomic approach to identify the potential targets of a vacuolar processing enzyme in the cellular context in tomato. Proteins were isolated from fruits of wild-type and RNAi tomato plants. More than 300 proteins were finally screened as significantly altered in the RNAi fruits. Based on Blast2go, a web-based bioinformatic tool that categorizes proteins according to their Gene Ontology (GO) annotations, the proteins experienced significant changes in abundance in the RNAi fruits were classified into fifteen functional categories.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Pulp

SUBMITTER: Guozheng Qin

LAB HEAD: Guozheng Qin

PROVIDER: PXD002980 | Pride | 2017-02-20

REPOSITORIES: Pride

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Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3.

Wang Weihao W Cai Jianghua J Wang Peiwen P Tian Shiping S Qin Guozheng G

Genome biology 20170307 1

<h4>Background</h4>Proteases represent one of the most abundant classes of enzymes in eukaryotes and are known to play key roles in many biological processes in plants. However, little is known about their functions in fruit ripening and disease resistance, which are unique to flowering plants and required for seed maturation and dispersal. Elucidating the genetic mechanisms of fruit ripening and disease resistance is an important goal given the biological and dietary significance of fruit.<h4>R ...[more]

PMID: 28270225

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