Proteomics

Dataset Information

0

SWATH-MS analysis of nuclear and cytoplasmic fractions of rat Sertoli cells

ABSTRACT: The Sertoli cells (Sc) of 5 days (infant) and 12 days (pubertal) old rat were isolated and cultured in triplicates. Nuclear and cytoplasmic fractionation was done for both the cases. All the four protein fractions (nuclear and cytoplasm of infant and pubertal Sc) were anaysed using Lc-MS/MS. SWATH analysis was done for all the four samples in biological and technical replicates. The objective was to quantity the proteins of all the samples with respect to each other at whole proteome level.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Sertoli Cell, Cell Culture

SUBMITTER: Kamal Mandal  

LAB HEAD: Dr. Subeer S. Majumdar

PROVIDER: PXD003056 | Pride | 2017-01-18

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
12_day_cytoplasm.zip Other
12_day_nucleus.zip Other
5_day_cytoplasm.zip Other
5_day_nucleus.zip Other
Rattus_norvegicus_cons_peakview_updatedRT_v2.txt Txt
Items per page:
1 - 5 of 5
altmetric image

Publications

An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation.

Mandal Kamal K   Bader Samuel L SL   Kumar Pankaj P   Malakar Dipankar D   Campbell David S DS   Pradhan Bhola Shankar BS   Sarkar Rajesh K RK   Wadhwa Neerja N   Sensharma Souvik S   Jain Vaibhav V   Moritz Robert L RL   Majumdar Subeer S SS  

DNA research : an international journal for rapid publication of reports on genes and genomes 20170401 2

Differential next-generation-omics approaches aid in the visualization of biological processes and pave the way for divulging important events and/or interactions leading to a functional output at cellular or systems level. To this end, we undertook an integrated Nextgen transcriptomics and proteomics approach to divulge differential gene expression of infant and pubertal rat Sertoli cells (Sc).Unlike, pubertal Sc, infant Sc are immature and fail to support spermatogenesis. We found exclusive as  ...[more]

Similar Datasets

Proteomics Comprehensive proteome profiling of nuclear and cytoplasmic fractions of rat Sertoli cells.
2017-01-18 | PXD003050 | Pride
Transcriptomics FSH and Testosterone treated Infant Monkey Sertoli cells vs. FSH and Testosterone treated pubertal Monkey Sertoli cell
2017-02-20 | GSE66129 | GEO
Transcriptomics FSH and Testosterone treated Infant Monkey Sertoli cells vs FSH and Testosterone treated pubertal Monkey Sertoli cell
2015-06-01 | GSE57937 | GEO
Proteomics Quantitative age-specific variability of plasma proteins in healthy neonates, children and adults
2017-03-27 | PXD005661 | Pride
Proteomics CPTAC High-grade serous ovarian carcinoma SWATH-MS
2020-07-14 | PXD010437 | Pride
Proteomics Inhibition of the autophagic protein ULK1 attenuates axonal degeneration in vitro and in vivo and modulates differential splicing
2020-04-30 | PXD011862 | Pride
Proteomics Quantitative proteomic analysis of the hemolymph from Bithynia siamensis goniomphalos infected with Opisthorchis viverrini
2016-10-17 | PXD004121 | Pride
Proteomics A primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics
2020-10-19 | PXD019542 | Pride
Proteomics,Multiomics Enterococcus faecalis V583, Part 1
2016-12-06 | PXD001576 | Pride
Proteomics Pediatric burn blister fluid LC-MS/MS SWATH
2018-12-21 | PXD011102 | Pride