Dataset Information

Gel-Free/Label-Free Proteomic Analysis Uncovers the Roles of Endoplasmic Reticulum of Soybean Root Tip under Flooding and Drought Stresses

ABSTRACT: As a stresses sensitive crop, this study will give some clues to enhance soybean tolerance of flooding and drought stresses. To uncover the response mechanisms occurring in the ER triggered by flooding and drought, ER proteomics was performed using gel-free/label-free proteomic approach. Besides, glycoproteomics was also producted to investigate the ER funtion in glycoprotein synthesis under stresses

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Root, Root Tip

DISEASE(S): Wounds And Injuries

SUBMITTER: Wang Xin

LAB HEAD: Setsuko KOMATSU

PROVIDER: PXD003397 | Pride | 2017-04-03

REPOSITORIES: Pride

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Gel-Free/Label-Free Proteomic Analysis of Endoplasmic Reticulum Proteins in Soybean Root Tips under Flooding and Drought Stresses.

Wang Xin X Komatsu Setsuko S

Journal of proteome research 20160602 7

Soybean is a widely cultivated crop; however, it is sensitive to flooding and drought stresses. The adverse environmental cues cause the endoplasmic reticulum (ER) stress due to accumulation of unfolded or misfolded proteins. To investigate the mechanisms in response to flooding and drought stresses, ER proteomics was performed in soybean root tips. The enzyme activity of NADH cytochrome c reductase was two-fold higher in the ER than other fractions, indicating that the ER was isolated with high ...[more]

PMID: 27224218

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Project description:Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. Root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially expressed proteins (DEPs). From RT1 tissues, 6,156, 7,687 and 7,699 proteins were quantified, and 296, 535 and 384 DEPs were identified in the SDT, D1W and D3W samples, respectively. From RT2 tissues, 7,382, 7,255 and 6,883 proteins were quantified, and 393, 587 and 321 proteins DEPs were identified in the SDT, D1W and D3W samples. Between RT1 and RT2 tissues, very few DEPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DEPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DEPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to choose the proteins (genes) as better defined targets for developing drought tolerant plants.

			Action	DRS
	GR140.mgf	Mgf
	GR140.msf	Msf
	GR140.pep.xml	Pepxml
	GR140.raw	Raw
	GR142D.mgf	Mgf