Dataset Information

Proteome profiling of Rhodobacter capsulatus wild type and a ΔmodABC mutant in response to molybdenum

ABSTRACT: The diazotrophic bacterium Rhodobacter capsulatus is able to synthesize two nitrogenases, a molybdenum-dependent and an alternative Mo-free iron-only nitrogenase, enabling growth with molecular dinitrogen (N2) as sole nitrogen source. The Mo response of the wild type and a mutant lacking the high-affinity molybdate transporter, ModABC, were analyzed by proteome profiling.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Rhodobacter Capsulatus (strain Atcc Baa-309 / Nbrc 16581 / Sb1003)

TISSUE(S): Nitrogen Fixing Cell

DISEASE(S): Disease Free

SUBMITTER: Sina Schäkermann

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD003123 | Pride | 2015-12-14

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Method.docx	Other
	PLGSoutput_Rc_438_with_Mo_Replicate1.zip	Other
	PLGSoutput_Rc_438_with_Mo_Replicate2.zip	Other
	PLGSoutput_Rc_438_with_Mo_Replicate3.zip	Other
	PLGSoutput_Rc_438_without_Mo_Replicate1.zip	Other

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Publications

Proteome Profiling of the Rhodobacter capsulatus Molybdenum Response Reveals a Role of IscN in Nitrogen Fixation by Fe-Nitrogenase.

Hoffmann Marie-Christine MC Wagner Eva E Langklotz Sina S Pfänder Yvonne Y Hött Sina S Bandow Julia E JE Masepohl Bernd B

Journal of bacteriology 20151207 4

<h4>Unlabelled</h4>Rhodobacter capsulatus is capable of synthesizing two nitrogenases, a molybdenum-dependent nitrogenase and an alternative Mo-free iron-only nitrogenase, enabling this diazotroph to grow with molecular dinitrogen (N2) as the sole nitrogen source. Here, the Mo responses of the wild type and of a mutant lacking ModABC, the high-affinity molybdate transporter, were examined by proteome profiling, Western analysis, epitope tagging, and lacZ reporter fusions. Many Mo-controlled prot ...[more]

PMID: 26644433

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