Dataset Information

Glycopeptide glycoforms analysis of mouse transferrin

ABSTRACT: Collagen type II-induced arthritis (CIA) is a disease, which includes inflammatory and autoimmune reactions. Spleen protein extracts were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analysis to identify proteins that were different in concentration ally expressed in CD38-KO and B6 WT mice with arthritis or with inflammation. Using multivariate analyses, in Col-II-immunized mice 20 differentially expressed spleen protein species were able to discriminate between WT and CD38-KO mice. Among them, multiple serotransferrin (Tf) species were identified. In contrast, in CFA/IFA-treated mice, the distinct protein profile, which discriminates between CD38-KO and WT mice, was unrelated with Tf. Unexpectedly, non-immunized CD38-KO mice showed a distinct proteome profile as compared with that in non-immunized WT mice, and again multiple protein species were identified as Tf. By using a LC-TOF-MS method to separate and detect Tf glycopeptide glycoforms in serum samples, differences in the Tf glycosylation pattern between CD38-KO and WT mice sera were found in both non-immunized and CIA+ mice. Data on Tf spots separated by 2-DE indicate differences in glycosylation related with NeuGc content. In summary, Tf changed significantly in its glycosylation pattern in mice with arthritis

INSTRUMENT(S): 6220 Time-of-Flight LC/MS

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Blood Serum

SUBMITTER: Albert Barroso

LAB HEAD: Victoria Sanz Nebot

PROVIDER: PXD003163 | Pride | 2015-12-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Glycopeptide_analysis_mTfSEARCH.xlsx	Xlsx
	Tf_bazo_2D_spot532_1-r002.mzXML	Mzxml
	Tf_bazo_2D_spot533_1-r002.mzXML	Mzxml
	Tf_bazo_2D_spot536_1-r002.mzXML	Mzxml
	Tf_bazo_2D_spot537_1-r002.mzXML	Mzxml

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Publications

Identification of multiple transferrin species in the spleen and serum from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with disease activity: The role of CD38.

Rosal-Vela A A Barroso A A Giménez E E García-Rodríguez S S Longobardo V V Postigo J J Iglesias M M Lario A A Merino J J Merino R R Zubiaur M M Sanz-Nebot V V Sancho J J

Journal of proteomics 20151127

<h4>Unlabelled</h4>Collagen type II-induced arthritis (CIA) is an inflammatory and autoimmune disease. Spleen protein extracts were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analysis to identify protein species that differ in abundance in CD38-KO versus B6 WT mice either with arthritis or with inflammation. Using multivariate analyses, in Col-II-immunized mice, 23 distinct spleen protein species were able to discriminate between WT and CD38-KO mice. Among them, several citrullinated proteins and ...[more]

PMID: 26639305

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