Dataset Information

Magnetic Transferrin Assay (MTA) as a novel approach for discovering serum exosome derived protein biomarkers in neurological diseases

ABSTRACT: We developed TF-based MNPs (TF-MNPs) to isolate blood-derived exosome in this study and isolated the blood-derived exosomes of patients with neuroinflammatory diseases, such as dementia, Parkinson’s disease and multiple sclerosis via this strategy and then evaluated the proteomic profiling of these exosomes to screen for a potential candidate biomarker. Blood was collected from 10 patients in each of the three disease groups. Over 800 serum exosome proteins were identified by highly sensitive nanoflow liquid chromatography-tandem mass spectrometry (LC−MS/MS).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

DISEASE(S): Multiple Sclerosis,Parkinson's Disease,Dementia

SUBMITTER: Hee-Sung Ahn

LAB HEAD: Kyunggon Kim

PROVIDER: PXD038555 | Pride | 2023-02-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20211104_Dementia1_59612490_E01.raw	Raw
	20211104_Dementia2_69698621_E02.raw	Raw
	20211104_Dementia3_75500426_E02.raw	Raw
	20211104_Dementia4_41142478_E02.raw	Raw
	20211104_Dementia5_88299429_E01.raw	Raw

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Publications

Magnetic transferrin nanoparticles (MTNs) assay as a novel isolation approach for exosomal biomarkers in neurological diseases.

Jang Yoon Ok YO Ahn Hee-Sung HS Dao Thuy Nguyen Thi TNT Hong JeongYeon J Shin Wangyong W Lim Young-Min YM Chung Sun Ju SJ Lee Jae-Hong JH Liu Huifang H Koo Bonhan B Kim Myoung Gyu MG Kim Kyunggon K Lee Eun-Jae EJ Shin Yong Y

Biomaterials research 20230216 1

<h4>Background</h4>Brain-derived exosomes released into the blood are considered a liquid biopsy to investigate the pathophysiological state, reflecting the aberrant heterogeneous pathways of pathological progression of the brain in neurological diseases. Brain-derived blood exosomes provide promising prospects for the diagnosis of neurological diseases, with exciting possibilities for the early and sensitive diagnosis of such diseases. However, the capability of traditional exosome isolation as ...[more]

PMID: 36797805

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