Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative Analysis of of Human Liver

ABSTRACT: Large scale proteomic analysis of human liver from 10 donors

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Jacek Wisniewski

LAB HEAD: Jacek R Wisniewski

PROVIDER: PXD003458 | Pride | 2018-04-23

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20141119_Qep7_JRW_SA_G10_2.raw	Raw
	20141119_Qep7_JRW_SA_G10_6.raw	Raw
	20141119_Qep7_JRW_SA_G10_T.raw	Raw
	20141119_Qep7_JRW_SA_G11_L2_141202175811.raw	Raw
	20141119_Qep7_JRW_SA_G11_L6.raw	Raw

Items per page:

1 - 5 of 62

Publications

Variability in Mass Spectrometry-based Quantification of Clinically Relevant Drug Transporters and Drug Metabolizing Enzymes.

Wegler Christine C Gaugaz Fabienne Z FZ Andersson Tommy B TB Wiśniewski Jacek R JR Busch Diana D Gröer Christian C Oswald Stefan S Norén Agneta A Weiss Frederik F Hammer Helen S HS Joos Thomas O TO Poetz Oliver O Achour Brahim B Rostami-Hodjegan Amin A van de Steeg Evita E Wortelboer Heleen M HM Artursson Per P

Molecular pharmaceutics 20170816 9

Many different methods are used for mass-spectrometry-based protein quantification in pharmacokinetics and systems pharmacology. It has not been established to what extent the results from these various methods are comparable. Here, we compared six different mass spectrometry-based proteomics methods by measuring the expression of clinically relevant drug transporters and metabolizing enzymes in human liver. Mean protein concentrations were in general quantified to similar levels by methods usin ...[more]

PMID: 28767254

Similar Datasets

Human liver homogenate, liver microsomes, low-speed centrifugation pellet and hepatocytes LC-MS/MS from 15 matched donors

Project description:LC-MS/MS-based label-free proteomics of human liver homogenate, human liver microsomes, and human hepatocytes from 15 matched donors used to compare and understand differences in two major in vitro systems used for drug metabolism studies

2021-03-29 | PXD014131 | Pride

Human liver cells proteome

Project description:The human liver functions through a complex interplay of parenchymal and non-parenchymal cells, which participate in performing many essential aspects of metabolism and secretion. Mass spectrometry-based proteomic analysis of intact tissue has improved the understanding of these processes, by providing an in-depth view of the human liver proteome. However, the predominance of parenchymal cells, i.e. hepatocytes, means that the total tissue proteome mainly reflects hepatocyte expression. In this study, we therefore used quantitative label-free proteomic analysis to analyze the proteomes of the major parenchymal and non-parenchymal cell types in the human liver, i.e. hepatocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatic stellate cells. We found that our data recapitulated specific functional differences between cell types, meaning that it can be used to reliably probe various aspects of cellular interplay. Thus, the data we provide constitutes a unique resource for exploring the human liver proteome at major cell type resolution.

2020-04-09 | PXD012615 | Pride

Identification of expression quantitative trait loci (eQTL) in human liver

2011-10-10 | GSE32504 | GEO

Identification of expression quantitative trait loci (eQTL) in human liver (SNP)

2012-07-03 | GSE39036 | GEO

Identification of expression quantitative trait loci (eQTL) in human liver

Project description:We performed genome-wide mRNA profiling of 149 human surgical liver samples from Caucasian donors with detailed medical documentation. The overall purpose of the study was to identify expression quantitative trait loci (eQTL) in human liver. 149 liver samples of Caucasian origin were included in the study on the analysis of RNA expression (Illumina Human_WG6_V2) and genotype (HumanHap_300_V1)

2011-10-09 | E-GEOD-32504 | biostudies-arrayexpress

Proteome Landscapes of the Human Colorectal Mucosa, and its Adenoma and Cancer

Project description:Adenomatous polyps in the colorectal tract are benign tumors with the potential to develop cancer. In this study we perform a large scale proteomic study of comparing the proteomes of colorectal enterocytes (N) and the cells of the adenoma (A) and cancer (C). Using formalin fixed and paraffin embedded clinical material, we identified on average 10,000 proteins per sample of the microdissected cells and established a quantitative protein repository of the disease. Statistical analysis revealed 2300, 1780, and 2161 significant alterations between N and A, C and A, and C and N, respectively. In this work we did not aim identification of novel biomarkers but focus on depiction of the proteome alterations underlying the disease. The extent of the assessed changes reflects a varied cell size, the composition of different subcellular components, and basic biological processes including the energy metabolism, plasma membrane transport, DNA replication and transcription.

2015-08-10 | PXD002137 | Pride

Identification of expression quantitative trait loci (eQTL) in human liver (SNP)

Project description:We genotyped 149 human surgical liver samples from Caucasian donors with detailed medical documentation. The overall purpose of the study was to identify expression quantitative trait loci (eQTL) in human liver..... 149 liver samples of Caucasian origin were included in the study on the analysis of RNA expression (Illumina Human_WG6_V2) and genotype (HumanHap_300_V2)

2012-07-02 | E-GEOD-39036 | biostudies-arrayexpress

Mouse liver regeneration quantitative proteomics

Project description:The liver is the only organ in mammals, which fully regenerates after injury. To identify novel regulators of liver regeneration, we performed quantitative large-scale proteomics analysis of subcellular fractions from normal versus regenerating mouse liver. Proteins of the ubiquitin-proteasome pathway were rapidly regulated by partial hepatectomy, with the ubiquitin ligase Nedd4-1 being among the top hits. Knock-down of Nedd4-1 in hepatocytes in vivo through nanoparticle-mediated delivery of siRNA caused severe liver damage after partial hepatectomy and impaired regeneration, resulting in liver failure. Mechanistically, we demonstrate that Nedd4-1 is required for efficient activation of Erk1/2 signaling by receptor tyrosine kinases involved in liver regeneration through inhibition of receptor internalization, thus controlling a major pro-mitogenic and cytoprotective signaling pathway in the regenerating liver. These results highlight the power of large-scale proteomics to identify key players in liver regeneration and the importance of posttranslational regulation of growth factor signaling in this process.

2017-09-13 | PXD004108 | Pride

TMT-16plex analysis of 25 individual human liver microsome and cytosol

Project description:Comprehensive proteomic analysis for human liver microsome and human liver cytosol from 25 individual donors focusing on drug metabolising enzymes with relative quantification with TMTpro.

2023-11-09 | PXD038055 | JPOST Repository

Transcriptomic study of human liver biopsies in obese patients with low and high liver fat

Project description:Non-alcoholic fatty liver disease (NAFLD) is a major risk factor leading to chronic liver disease and type 2 diabetes. In this study liver metabolic activity and functionality in NAFLD was charted by integrating global transcriptomic data within a genome-scale model of human metabolism. Gene expression was measured in the human liver of eight subjects with extreme steatosis diagnosed with NAFLD (liver fat % range 20-80%), and eight healthy, but obese subjects with low liver fat content (liver fat % range 0-10%). This dataset is part of the TransQST collection.

2016-06-01 | E-MTAB-4231 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data