Proteomics

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Proteome Landscapes of the Human Colorectal Mucosa, and its Adenoma and Cancer

ABSTRACT: Adenomatous polyps in the colorectal tract are benign tumors with the potential to develop cancer. In this study we perform a large scale proteomic study of comparing the proteomes of colorectal enterocytes (N) and the cells of the adenoma (A) and cancer (C). Using formalin fixed and paraffin embedded clinical material, we identified on average 10,000 proteins per sample of the microdissected cells and established a quantitative protein repository of the disease. Statistical analysis revealed 2300, 1780, and 2161 significant alterations between N and A, C and A, and C and N, respectively. In this work we did not aim identification of novel biomarkers but focus on depiction of the proteome alterations underlying the disease. The extent of the assessed changes reflects a varied cell size, the composition of different subcellular components, and basic biological processes including the energy metabolism, plasma membrane transport, DNA replication and transcription.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Mario Oroshi  

LAB HEAD: Jacek R Wisniewski

PROVIDER: PXD002137 | Pride | 2015-08-10

REPOSITORIES: Pride

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20111205_EXQ6_JRW_SA_ADE1_L_01.raw Raw
20111205_EXQ6_JRW_SA_ADE1_L_02.raw Raw
20111205_EXQ6_JRW_SA_ADE1_L_03.raw Raw
20111205_EXQ6_JRW_SA_ADE1_L_04.raw Raw
20111205_EXQ6_JRW_SA_ADE1_T_05.raw Raw
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Publications

Absolute Proteome Analysis of Colorectal Mucosa, Adenoma, and Cancer Reveals Drastic Changes in Fatty Acid Metabolism and Plasma Membrane Transporters.

Wiśniewski Jacek R JR   Duś-Szachniewicz Kamila K   Ostasiewicz Paweł P   Ziółkowski Piotr P   Rakus Dariusz D   Rakus Dariusz D   Mann Matthias M  

Journal of proteome research 20150817 9

Colorectal cancer is a leading cause of cancer-related death. It develops from normal enterocytes, through a benign adenoma stage, into the cancer and finally into the metastatic form. We previously compared the proteomes of normal colorectal enterocytes, cancer and nodal metastasis to a depth of 8100 proteins and found extensive quantitative remodeling between normal and cancer tissues but not cancer and metastasis (Wiśniewski et al. PMID 22968445). Here we utilize advances in the proteomic wor  ...[more]

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