Dataset Information

Protein expression in tomato tissues as a function of Al3+ stress (Suping_Exp4)

ABSTRACT: The goal of this experiment is to identify the changes in protein expression induced by Al treatment with a view towards developing a molecular level understanding of Al tolerance in an important crop species. In this particular experiment the focus was on protein expression in tomato embryo. Tomato (Solanum lycopersicum ‘Micro-Tom’) plants were treated in Magnavaca’s solution (pH 4.5) supplemented with 50 µM AlK(SO4)2·12H2O providing 7.5 µM Al3+ activity over a four-month period beginning at the emergence of the first flower buds and ending when a few leaves started to turn yellow. Proteins from root, leaf, embryo and seed-coat tissues were identified using a 8-plex iTRAQ labeling strategy followed by a two-dimensional (high- and low-pH) chromatographic separation, and final generation of MS/MS spectra of tryptic peptides on an LTQ-Orbitrap Elite-mass spectrometer

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Embryo

SUBMITTER: Ted Thannhauser

LAB HEAD: Ted Thannhauser

PROVIDER: PXD003601 | Pride | 2016-04-25

REPOSITORIES: Pride

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Publications

Proteome Modification in Tomato Plants upon Long-Term Aluminum Treatment.

Zhou Suping S Okekeogbu Ikenna I Sangireddy Sasikiran S Ye Zhujia Z Li Hui H Bhatti Sarabjit S Hui Dafeng D McDonald Daniel W DW Yang Yong Y Giri Shree S Howe Kevin J KJ Fish Tara T Thannhauser Theodore W TW

Journal of proteome research 20160418 5

This study aimed to identify the aluminum (Al)-induced proteomes in tomato (Solanum lycopersicum, "Micro-Tom") after long-term exposure to the stress factor. Plants were treated in Magnavaca's solution (pH 4.5) supplemented with 7.5 μM Al(3+) ion activity over a 4 month period beginning at the emergence of flower buds and ending when the lower mature leaves started to turn yellow. Proteomes were identified using a 8-plex isobaric tags for relative and absolute quantification (iTRAQ) labeling str ...[more]

PMID: 27052409

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			Action	DRS
	F399569.mzid.gz	Mzid
	F399569.pride.mztab.gz	Mztab
	Suping_Exp4_Tomato.mgf	Mgf
	Suping_Exp4_Tomato.pride.mgf.gz	Mgf
	Suping_Exp4_Tomato_1.raw	Raw