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Downstream targets of Beauveria bassiana gelsolin during blastospore development

ABSTRACT: Comparative proteomics, between the wild type and delta BbGel1 mutant strains grown in liquid media, is performed to explore the downstream targets of Beauveria bassiana gelsolin during balstospore development .

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Beauveria Bassiana (strain Arsef 2860) (white Muscardine Disease Fungus) (tritirachium Shiotae)

TISSUE(S): Cell Culture

SUBMITTER: Sheng-hua Ying

LAB HEAD: Sheng-hua Ying

PROVIDER: PXD003617 | Pride | 2016-09-26

REPOSITORIES: Pride

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Publications

The cellular proteome is affected by a gelsolin (BbGEL1) during morphological transitions in aerobic surface versus liquid growth in the entomopathogenic fungus Beauveria bassiana.

He Pu-Hong PH Dong Wei-Xia WX Chu Xin-Ling XL Feng Ming-Guang MG Ying Sheng-Hua SH

Environmental microbiology 20160913 11

The gelsolin superfamily includes seven protein members: gelsolin, villin, adseverin, CapG, advillin, supervillin and flightless I. The gelsolin proteins are actin-binding proteins that contain three or six gelsolin-like domains, and they play important roles in remodelling actin dynamics and cellular processes in eukaryotes. The entomopathogenic fungus Beauveria bassiana expresses a unique CapG protein (BbGEL1) that contains three gelsolin-like domains. BbGEL1p is associated with actin during m ...[more]

PMID: 27554994

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Analyzing downstream targets of Beauveria bassiana gelsolin duing conidal development

Project description:Comparative proteomics, between the wild type and delta BbGel1 strains grown on the surface of culture media, is performed to explore the downstream targets of Beauveria bassiana gelsolin during conidiation .

2016-09-26 | PXD003618 | Pride

Quantitative analysis of proteome reveals the roles of Rpd3 and Sir2 in asexual development, virulence and multiple stress responses in Beauveria bassiana.

Project description:Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylation of histone H3 and H4. We unveil the role of Rpd3 in asexual development and show that a ΔRpd3 mutant strain has severe growth defects and is defective in conidiophores and blastospores development. Proteomic analyses indicated that Rpd3 functions in asexual development and virulence and multiple stress responses via broad genetic pathways in B. bassiana. Therefore, we conclude that BbRpd3 is vital for asexual development, virulence and multiple stress responses in Beauveria bassiana.

2021-08-12 | PXD006713 | Pride

Quantitative analysis of acetylome reveals the roles of Rpd3 and Sir2 in asexual development, virulence and multiple stress responses in Beauveria bassiana.

Project description:Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylation of histone H3 and H4. We unveil the role of Rpd3 in asexual development and show that a ΔRpd3 mutant strain has severe growth defects and is defective in conidiophores and blastospores development. Acetylomic analyses indicated that Rpd3 functions in asexual development and virulence and multiple stress responses via broad genetic pathways in B. bassiana. Therefore, we conclude that BbRpd3 is vital for asexual development, virulence and multiple stress responses in Beauveria bassiana.

2021-08-12 | PXD006714 | Pride

Genome-wide gene expression analysis of HCT116 cells subjected to stable gelsolin overexpression

Project description:Analysis of gene expression levels altered by stable gelsolin overexpression. Results provide important information of the modulation of various genes by gelsolin.

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Transcription profiling of mouse gelsolin +/+ and gelsolin -/- mutants

Project description:Hippocampi from gelsolin +/+ and gelsolin -/- mice were used for transcription profiling.

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the secretome of Pseudoalteromonas prydzensis ACAM 620

Project description:Chitin-degrading enzymes secreted by Pseudoalteromonas prydzensis ACAM 620 were identified by proteomic analysis.

2022-09-14 | PXD031176 | Pride

Genome-wide gene expression analysis of HCT116 cells subjected to stable gelsolin overexpression

Project description:Analysis of gene expression levels altered by stable gelsolin overexpression. Results provide important information of the modulation of various genes by gelsolin. Total RNA obtained from stable gelsolin-overexpressing HCT116 clones (C1, C2, C3, C8) as well as emtpy vector control HCT116 cell lines (V4, V5)

2014-03-01 | E-GEOD-36588 | biostudies-arrayexpress

Selective control of metabolism by the GCN2 eIF2 kinase pathway in Saccharomyces cerevisiae

Project description:When eukaryotic cells are deprived of amino acids, uncharged tRNAs accumulate and activate the conserved GCN2 protein kinase. We examine how yeast growth and tRNA charging or aminoacylation is affected during amino acid depletion in the presence and absence of GCN2. tRNA charging is measured using a microarray technique which allows for simultaneous measurement of all cytosolic tRNAs. A fully prototrophic and its isogenic GCN2 deletion strain were used. We measured relative tRNA charging levels in yeast strains with an intact and deleted GCN2.

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Steady state growth under inorganic carbon limitation increases energy consumption for maintenance and enhances nitrous oxide production in Nitrosomonas europaea

Project description:Nitrosomonas europaeais a chemolithoautotrophic bacterium that oxidizes ammonia (NH3) to obtain energy for growth on carbon dioxide (CO2), and can also produce nitrous oxide (N2O), a greenhouse gas. We interrogated the growth, physiological, and transcriptome responses ofN. europaeato replete (> 5.2 mM) and limited inorganic carbon (IC) provided by either 1.0 mM or 0.2 mM sodium carbonate (Na2CO3) supplemented with atmospheric CO2 IC-limited cultures oxidized 25 to 58% of available NH3to nitrite, depending on dilution rate and Na2CO3concentration. IC limitation resulted in a 2.3-fold increase in cellular maintenance energy requirements compared to NH3-limited cultures. Rates of N2O production increased 2.5- and 6.3-fold under the two IC-limited conditions increasing the percentage of oxidized NH3-N being transformed to N2O-N from 0.5% (replete) up to 4.4% (0.2 mM Na2CO3). Transcriptome analysis showed differential expression (pâ¤ 0.05) of 488 genes (20% of inventory) between replete and IC-limited conditions, but few differences were detected between the two IC-limiting treatments. IC-limited conditions resulted in decreased expression of ammonium/ammonia transporter and ammonia monooxygenase subunits, and increased expression of genes involved in C1 metabolism including RuBisCO (cbbgene cluster), carbonic anhydrase, folate-linked metabolism of C1 moieties, and putative C salvage due to oxygenase activity of RuBisCO. Increased expression of nitrite reductase (gene cluster NE0924-0927) correlated with increased production of N2O. Together, these data suggest thatN. europaeaadapts physiologically during IC-limited steady state growth, which leads to uncoupling of NH3oxidation from growth and increased N2O production. Transcriptome responses of N.Â europaea in 60Â mM NH4+ to suboptimum levels of carbonate (1.0 mM and 0.2 mM) in continuous steady-state culture in a bioreactor.

2016-03-28 | E-GEOD-70988 | biostudies-arrayexpress

A Tandem Mass Tag (TMT) proteomic analysis during the early phase of experimental pancreatitis

Project description:We performed in a rat pancreatitic model a quantitative shotgun proteomic profiling of the soluble and whole membrane fractions from the pancreas during the early phase of cerulein (Cer)-induced AP compared to controls. Three control and three pancreatitic samples from both the soluble and the whole membrane fractions were analysed in two independent TMT6plex experiments. After trypsin digestion and TMT labelling, the peptide mixtures were fractionated into 12 fractions by off-gel electrophoresis. ESI LTQ-OT MS was performed on a LTQ Orbitrap velos equipped with a NanoAcquity system from Waters. MS data were analysed for protein identifications using EasyProt (v2.3) and peak lists were generated into .mgf format with EasyProtConv (CID/HCD merging was used to improve peptide identification and quantification). Searches were conducted against UniProt Swiss-Prot database (UniProtKB release 2014_10 of Oct, 29, 2014), specifying Rattus norvegicus taxonomy. Only proteins with at least two unique peptide sequences and a false discovery rate (FDR) ≤ 1 % were selected for further quantification. Proteins were clustered based on shared peptides indistinguishable by MS. Quantification was conducted using Isobar R package (v.1.9.3.2). We identified 997 proteins, of which 353 were significantly differentially expressed (22, 256 or 55 in both, the soluble or the membrane fractions, respectively).

2018-05-22 | PXD007096 | Pride

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