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So ce56 secretome LC-MS/MS

ABSTRACT: In this work, the secretome of the aerobic soil bacterium Sorangium cellulosum So ce56 was studied and the main hemicellulases for the degradation of arabinoxylan, avicel, wheat straw and oat spelts xylan were determined. For this purpose, bacterial cultures were set in triplicate for each substrate as well as for the basic medium CNS, which contained no lignocellulose substrate. Samples from the supernatants were taken after 5, 10 and 15 days of cultivation. After LC-MS/MS analysis, the secreted hemicellulases were identified and relatively quantified.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sorangium Cellulosum (strain So Ce56) (polyangium Cellulosum (strain So Ce56))

TISSUE(S): Secretion

SUBMITTER: Alexander Graf

LAB HEAD: Mueller-Roeber

PROVIDER: PXD003639 | Pride | 2022-03-02

REPOSITORIES: Pride

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Project description:Background: Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant substrates. Brown-rot strain produces carbohydrate-active enzymes involved in the degradation of lignocellulosic materials, along with a non-enzymatic mechanism, via Fenton reaction. Differences in the lignocellulose metabolism occurring even among closely related brown-rots are not completely understood, bringing attention to a multi-omics study of brown-rot L. sulphureus. Results: To evidence the oxidative-hydrolytic mechanism, the Laetiporus sulphureus ATCC 52600 genome was sequenced and the response to lignocellulosic substrates was analyzed by transcriptomics and proteomics. The transcriptomic profile in response to a short cultivation period on in natura sugarcane bagasse revealed 128 out of 12,802 upregulated transcripts. The high upregulated transcripts included a set of redox enzymes along with hemicellulases. The exoproteome in response to extended-time cultivation with Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus grandis revealed 121 proteins. Contrasting to the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 proteins upregulated relative to glucose. The secretome produced on sugarcane bagasse was evaluated in the saccharification of pretreated sugarcane straw by supplementing a commercial cocktail. Additionally, growth analysis revealed that L. sulphureus ATCC 52600 has higher efficiency to assimilate glucose than other mono and disaccharides. Conclusion: This study shows the singularity of L. sulphureus ATCC 52600 relative to other Polyporales brown-rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omic analysis reinforces the oxidative-hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step.

			Action	DRS
	Samplesinfo.xlsx	Xlsx
	maria2in20_10.RAW	Raw
	maria2in20_10.mgf	Mgf
	maria2in20_10.pride.mgf.gz	Mgf
	maria2in20_11.RAW	Raw

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So ce56 secretome LC-MS/MS

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