Proteomic analysis of plasma membrane and tonoplast from the leaves of a mangrove plant Avicennia officinalis

ABSTRACT: We used gel electrophoresis coupled with LC-MS based proteomics to identify key transport proteins in the plasma membrane (PM) and tonoplast fractions of Avicennia officinalis leaves. This was part of our attempts to understand salt tolerance and secretion in mangrove plant species. PM and tonoplast proteins were purified using two-aqueous phase partitioning and density gradient centrifugation, respectively. Thirty of the 154 PM proteins and 31 of the 118 tonoplast proteins identified were predicted to have transmembrane domains. About 90% of the identified proteins could be classified based on their functions. The major classes of proteins were predicted to be involved in transport, metabolic processes, signal transduction and defense /stress response, while a few of the proteins were predicted to be involved in membrane trafficking. The main classes of transporter proteins identified included H+-ATPases, ATP-binding cassette transporters (ABC) and aquaporins, all of which could play a role in salt secretion. These data will serve as the baseline membrane proteomic dataset for Avicennia species. Further, this information can contribute to future studies on understanding the mechanism of salt tolerance in halophytes in addition to salt secretion in mangroves.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Avicennia

TISSUE(S): Leaf

SUBMITTER: Qingsong Lin  

LAB HEAD: Assistant Professor Lin Qingsong

PROVIDER: PXD000837 | Pride | 2014-11-18

REPOSITORIES: Pride