Dataset Information

Quantitative proteome profiling of Leishmania donovani after hypericin treatment

ABSTRACT: Hypericin, a novel inhibitor of spermidine synthase of Leishmania donovani, has been found to cause necrotic like death of the parasite. Hypericin treatment has indicated the importance of spermidine in pathways other than redox metabolism of Leishmania parasite. We have used label free quantitative proteomics approach to compare the alteration in expression of proteins in untreated and hypericin treated Leishmania promastigotes.

INSTRUMENT(S): LTQ Orbitap Discovery

ORGANISM(S): Leishmania Donovani

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Visceral Leishmaniasis

SUBMITTER: Shalini Singh

LAB HEAD: Professsor Vikash Kumar Dubey

PROVIDER: PXD003815 | Pride | 2016-04-21

REPOSITORIES: Pride

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Quantitative Proteome Analysis of Leishmania donovani under Spermidine Starvation.

Singh Shalini S Dubey Vikash Kumar VK

PloS one 20160428 4

We have earlier reported antileishmanial activity of hypericin by spermidine starvation. In the current report, we have used label free proteome quantitation approach to identify differentially modulated proteins after hypericin treatment. A total of 141 proteins were found to be differentially regulated with ANOVA P value less than 0.05 in hypericin treated Leishmania promastigotes. Differentially modulated proteins have been broadly classified under nine major categories. Increase in ribosomal ...[more]

PMID: 27123864

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