Proteomics

Dataset Information

0

Analysis of the glycoproteins present on Fasciola hepatica surface tegument


ABSTRACT: Fasciola hepatica, commonly known as liver fluke, is a trematode which causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica is a complex metabolically active biological matrix that is continually exposed to the host immune system. It is highly glycosylated and parasite-derived immunogenic oligosaccharide motifs and glycoproteins are currently being investigated as novel vaccine candidates. We selectively enriched for FhTeg mannosylated glycoprotein subsets using lectin affinity chromatography and identified 369 proteins by mass spectrometric analysis.

INSTRUMENT(S): LTQ

ORGANISM(S): Fasciola Hepatica (liver Fluke)

TISSUE(S): Integument

SUBMITTER: Krystyna Cwiklinski  

LAB HEAD: Sandra O'Neill

PROVIDER: PXD003911 | Pride | 2016-08-03

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
Ingel19182.mgf Mgf
Ingel19182.pride.mgf.gz Mgf
Ingel19182.wiff Wiff
Ingel19182.wiff.scan Wiff
Ingel19182_MGFPeaklist (F060175).mzid_Ingel19182_MGFPeaklist_(F060175).pride.mgf.gz Mzid
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Publications

Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host.

Ravidà Alessandra A   Cwiklinski Krystyna K   Aldridge Allison M AM   Clarke Paul P   Thompson Roisin R   Gerlach Jared Q JQ   Kilcoyne Michelle M   Hokke Cornelis H CH   Dalton John P JP   O'Neill Sandra M SM  

Molecular & cellular proteomics : MCP 20160727 10


Fasciola hepatica, commonly known as liver fluke, is a trematode that causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica (FhTeg) is a complex metabolically active biological matrix that is continually exposed to the host immune system and therefore makes a good vaccine target. F. hepatica tegumental coat is highly glycosylated and helminth-derived immunogenic oligosaccharide motifs and glycoproteins are currently being investigated as novel vaccine candidates. T  ...[more]

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