Dataset Information

Identification of Proteins Sensitive to Thermal Stress in Human Neuroblastoma and Glioma Cell Lines

ABSTRACT: Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in cells of neuronal (SH-SY5Y) and glial (CCF-STTG1) lineage, in conjunction with detergent extraction and sedimentation followed by LC-MS/MS proteomic approaches, we sought to identify human proteins that lose solubility upon heat-shock.

INSTRUMENT(S): QSTAR

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Guilian Xu

LAB HEAD: David R. Borchelt

PROVIDER: PXD003914 | Pride | 2016-04-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20070817STTGDOCP37_1.wiff	Wiff
	20070817STTGDOCP37_2.wiff	Wiff
	20070817STTGDOCP37_3.wiff	Wiff
	20070817STTGDOCP37_4.wiff	Wiff
	20070817STTGDOCP37_5.wiff	Wiff

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Publications

Identification of proteins sensitive to thermal stress in human neuroblastoma and glioma cell lines.

Xu Guilian G Stevens Stanley M SM Kobeissy Firas F Brown Hilda H McClung Scott S Gold Mark S MS Borchelt David R DR

PloS one 20121108 11

Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in cells of neuronal (SH-SY5Y) and glial (CCF-STTG1) lineage, in conjunction with detergent extraction and sedimentation followed by LC-MS/MS proteomic approaches, we sought to identify human proteins that lose solubility upon heat-shock. The two ...[more]

PMID: 23145051

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