Dataset Information

ELAV proteomics after brain ischemia and reperfusion in the rat

ABSTRACT: We investigated mRNA regulatory proteins of the ELAV family following 10 min global brain ischemia and 8 hrs reperfusion (8R) or non-ischemic controls (NIC) in rat hippocampal CA1 and CA3. There were three main experiments: (1) Shot-gun proteomics of polysome pellets from NIC and 8R CA1 and CA3, (2) Shot-gun proteomics of ELAV immunoprecipitate eluents from NIC and 8R CA1 and CA3, and (3) Proteomics of ELAV antisera-reactive bands excised from nitrocellulose following ELAV Western blot.

OTHER RELATED OMICS DATASETS IN: PRJNA324171

INSTRUMENT(S): QSTAR

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Hippocampal Pyramidal Neuron

DISEASE(S): Brain Ischemia

SUBMITTER: Donald DeGracia

LAB HEAD: Donald J. DeGracia

PROVIDER: PXD004078 | Pride | 2016-07-07

REPOSITORIES: Pride

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Publications

Embryonic lethal abnormal vision proteins and adenine and uridine-rich element mRNAs after global cerebral ischemia and reperfusion in the rat.

Wang Haihui H Tri Anggraini Fika F Chen Xuequn X DeGracia Donald J DJ

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20160101 4

Prolonged translation arrest correlates with delayed neuronal death of hippocampal CA1 neurons following global cerebral ischemia and reperfusion. Many previous studies investigated ribosome molecular biology, but mRNA regulatory mechanisms after brain ischemia have been less studied. Here we investigated the embryonic lethal abnormal vision/Hu isoforms HuR, HuB, HuC, and HuD, as well as expression of mRNAs containing adenine and rich uridine elements following global ischemia in rat brain. Prot ...[more]

PMID: 27381823

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			Action	DRS
	HuRIP05302014.wiff	Wiff
	HuRIP06092014.wiff	Wiff
	HuRIP06102014.wiff	Wiff
	HuRIPN1106092014.dat	Other
	HuRIPN1206092014.dat	Other