Dataset Information

Allele-Specific Protein Abundance in Human Brain

ABSTRACT: We describe a method for identifying peptides that result from missense changes and identify peptides among 2 human brains that would have otherwise not been detected. Next, we use this data to estimate of allele-specific protein abundance in human brain for an average per individual, and to estimate apolipoprotein E allele specific abundance in human brain across individuals. Finally, we estimate the heritability of allele-specific protein abundance.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Duc Duong

LAB HEAD: NIcholas Seyfried

PROVIDER: PXD004143 | Pride | 2017-07-31

REPOSITORIES: pride

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Publications

Integrating Next-Generation Genomic Sequencing and Mass Spectrometry To Estimate Allele-Specific Protein Abundance in Human Brain.

Wingo Thomas S TS Duong Duc M DM Zhou Maotian M Dammer Eric B EB Wu Hao H Cutler David J DJ Lah James J JJ Levey Allan I AI Seyfried Nicholas T NT

Journal of proteome research 20170809 9

Gene expression contributes to phenotypic traits and human disease. To date, comparatively less is known about regulators of protein abundance, which is also under genetic control and likely influences clinical phenotypes. However, identifying and quantifying allele-specific protein abundance by bottom-up proteomics is challenging since single nucleotide variants (SNVs) that alter protein sequence are not considered in standard human protein databases. To address this, we developed the GenPro so ...[more]

PMID: 28691493

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			Action	DRS
	E05-90.fasta	Fasta
	OS02-252.fasta	Fasta
	OS02252_01_LyscTrypsin.raw	Raw
	e0590_01_LysConly.raw	Raw
	e0590_lt_01_LyscTrypsin.raw	Raw