Unknown,Transcriptomics,Genomics,Proteomics

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An PWI- and RRM Motif-Containing Protein Is Critical for Pre-mRNA Splicing and ABA Responses in Arabidopsis


ABSTRACT: We used RNA-seq platform to determine role of a splicing factor RBM25 in regulation of gene expression and pre-mRNA splicing. We found that a loss-of-function allele of RBM25, rbm25-1, causes up- and down-regulation of a large number of genes. We further found that the rbm25-1 mutation results in defects in altenative splicing of transcripts of many genes including signal transduction components in stress responses. Examination of mRNA levels in bulked individual wild type and rbm25-1 mutant seedlings before and after ABA treatment.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jianhua Zhu 

PROVIDER: E-GEOD-66737 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses.

Zhan Xiangqiang X   Qian Bilian B   Cao Fengqiu F   Wu Wenwu W   Yang Lan L   Guan Qingmei Q   Gu Xianbin X   Wang Pengcheng P   Okusolubo Temiloluwa A TA   Dunn Stephanie L SL   Zhu Jian-Kang JK   Zhu Jianhua J  

Nature communications 20150925


The phytohormone abscisic acid (ABA) is important for growth, development and stress responses in plants. Recent research has identified ABA receptors and signalling components that regulate seed germination and stomatal closure. However, proteins that regulate ABA signalling remain poorly understood. Here we use a forward-genetic screen to identify rbm25-1 and rbm25-2, two Arabidopsis mutants with increased sensitivity to growth inhibition by ABA. Using RNA-seq, we found that RBM25 controls the  ...[more]

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