Dataset Information

Degradation of C30 - Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities

ABSTRACT: A significant part of the heavier petroleum fraction resulting from offshore oil-spills sinks to the deep-sea. Its fate and biodegradation by microbial communities is unclear. In particular, the physiological and metabolic features of hydrostatic pressure (HP) adapted oil-degraders have been neglected. In this study, hydrocarbon-free sediment from 1km below surface water (bsl) was incubated at 0.1, 10 and 20MPa (equivalent to surface waters, 1 and 2km bsl) using triacontane (C30) as sole carbon source for a 3-month enrichment period. HP strongly impacted biodegration, as it selected for microbial communities with small cells, high O2 respiration and nutrients requirements, but low biomass and C30-degradation yields. The alkane-degrading metaproteome linked to β-oxidation was detected but its expression was reduced under HP contrary to several housekeeping genes. This was reflected in the enriched communities, as atmospheric pressure was dominated by hydrocarbonoclastic bacteria while non-specialized or previously unrecognized oil-degrading genera were enriched under HP.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Escherichia Coli

SUBMITTER: Robert Heyer

LAB HEAD: DIrk Benndorf

PROVIDER: PXD004328 | Pride | 2018-12-19

REPOSITORIES: Pride

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Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities.

Scoma Alberto A Heyer Robert R Rifai Ridwan R Dandyk Christian C Marshall Ian I Kerckhof Frederiek-Maarten FM Marietou Angeliki A Boshker Henricus T S HTS Meysman Filip J R FJR Malmos Kirsten G KG Vosegaard Thomas T Vermeir Pieter P Banat Ibrahim M IM Benndorf Dirk D Boon Nico N

The ISME journal 20181212 4

Petroleum hydrocarbons reach the deep-sea following natural and anthropogenic factors. The process by which they enter deep-sea microbial food webs and impact the biogeochemical cycling of carbon and other elements is unclear. Hydrostatic pressure (HP) is a distinctive parameter of the deep sea, although rarely investigated. Whether HP alone affects the assembly and activity of oil-degrading communities remains to be resolved. Here we have demonstrated that hydrocarbon degradation in deep-sea mi ...[more]

PMID: 30542078

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			Action	DRS
	01MPa_10d_M1.dat	Other
	01MPa_10d_M1.mgf	Mgf
	01MPa_10d_M1.raw	Raw
	01MPa_10d_M2.dat	Other
	01MPa_10d_M2.mgf	Mgf