Dataset Information

Proteomic analysis uncovers common effects of IFN-γ and IL-27 on antigen presentation machinery components in human cancer cells

ABSTRACT: IL-27, a member of the IL-12- family of cytokines, has shown anti-tumor activity in several pre-clinical models due to anti-proliferative, anti-angiogenic and immune-enhancing effects. On the other hand, IL-27 demonstrated immune-regulatory activities and inhibition of auto-immunity in mouse models. Also, we reported that IL-27, similar to IFN-, induces the expression of IL-18BP, IDO and PD-L1 immune-regulatory molecules in human cancer cells. Here, a proteomic analysis reveals that IL-27 and IFN- display a broad overlap of functions on human ovarian cancer cells. Indeed, among 990 proteins modulated by either cytokine treatment in SKOV3 cells, 814 showed a concordant modulation by both cytokines, while a smaller number (176) were differentially modulated. The most up-regulated proteins were common to both IFN-γ and IL-27. In addition, functional analysis of IL-27- regulated protein networks highlighted pathways of interferon signaling and regulation, antigen presentation, protection from natural killer cell-mediated cytotoxicity, regulation of protein polyubiquitination and proteasome, aminoacid catabolism and regulation of viral protein levels.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Andrea Petretto

LAB HEAD: Andrea Petretto

PROVIDER: PXD004419 | Pride | 2017-05-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	FerriniOT_1214_OC316_CT_1_7.raw	Raw
	FerriniOT_1214_OC316_CT_4_8.raw	Raw
	FerriniOT_1214_OC316_CT_7_9.raw	Raw
	FerriniOT_1214_OC316_IFNg_3_33.raw	Raw
	FerriniOT_1214_OC316_IFNg_6_34.raw	Raw

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Publications

Proteomic analysis uncovers common effects of IFN-γ and IL-27 on the HLA class I antigen presentation machinery in human cancer cells.

Petretto Andrea A Carbotti Grazia G Inglese Elvira E Lavarello Chiara C Pistillo Maria Pia MP Rigo Valentina V Croce Michela M Longo Luca L Martini Stefania S Vacca Paola P Ferrini Silvano S Fabbi Marina M

Oncotarget 20161101 45

IL-27, a member of the IL-12-family of cytokines, has shown anti-tumor activity in several pre-clinical models due to anti-proliferative, anti-angiogenic and immune-enhancing effects. On the other hand, IL-27 demonstrated immune regulatory activities and inhibition of auto-immunity in mouse models. Also, we reported that IL-27, similar to IFN-γ, induces the expression of IL-18BP, IDO and PD-L1 immune regulatory molecules in human cancer cells. Here, a proteomic analysis reveals that IL-27 and IF ...[more]

PMID: 27683036

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