Dataset Information

Genome-wide functional analysis of AreA and AreB

ABSTRACT: "Background: The ascomycete Fusarium fujikuroi causes “bakanae” disease of rice due to its ability to produce gibberellins (GA), but is also known for producing a broad spectrum of other secondary metabolites, (SM) such as pigments and harmful mycotoxins. Biosynthesis of multiple SM is regulated by nitrogen availability. The global GATA transcription factors (TF) AreA and AreB were shown to play key roles in this nitrogen-dependent regulation. Recently, we have shown that AreA activates the transcription of AREB, but not vice versa. Both regulators interact in the nucleus, indicating a complex regulatory interplay between both regulators. Results: We performed transcriptome and proteome analyses of the wild type and ΔAREA and ΔAREB mutants under nitrogen limiting and sufficient conditions. Microarray analysis revealed that each transcription factor regulates a total set of about 4,200 genes under nitrogen limiting conditions. Gene enrichment analysis revealed that significantly up- and down-regulated genes belong to functional classes of primary/secondary metabolism, transporters, cell defense/virulence and transcription. Under nitrogen excess, AreA is not active as a transcriptional regulator, while AreB affects expression of about 4,400 genes. Both transcription factors regulate a common set of about 2,100 genes, often coordinately either as positive or as negative regulators. Our proteomics analysis identified 446 AreA-regulated and 386 AreB-regulated proteins under nitrogen limitation and 138 AreA-regulated and 447 AreB-regulated proteins under nitrogen sufficiency, respectively. Comparison of the proteome and transcriptome data indicates that both GATA-factors not only regulate expression of target genes but also affect protein abundance by post-transcriptional regulations, for example on enzymes involved in glutamate cycling, as well as histone acetylation at some of the target SM gene clusters. Conclusion: This study provides novel insights into the AreA- and AreB-mediated regulation network on a cellular scale. Microarray analysis clearly demonstrated that both GATA-factors are major regulators of alternate nitrogen assimilatory pathways and secondary metabolism. Moreover, proteome analysis of the AREA and AREB deletion mutants showed that both TF are global players interconnecting carbon and nitrogen regulation. They mainly act as mutualistic, but also antagonistic regulators and operate by transcriptional and post-transcriptional regulations and epigenetic modifications." (Abstract taken from manuscript)

OTHER RELATED OMICS DATASETS IN: PRJNA319032

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Fusarium Fujikuroi Imi 58289

TISSUE(S): Cell Culture

SUBMITTER: Johannes Leufken

LAB HEAD: Prof. Dr. Bettina Tudzynski

PROVIDER: PXD004664 | Pride | 2017-04-26

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	AP_15052014_LTQ_S1_100pro_GlnA_pH11_140520191626.mzML.gz	Mzml
	AP_15052014_LTQ_S1_100pro_GlnA_pH3.mzML.gz	Mzml
	AP_15052014_LTQ_S1_100pro_GlnA_pH4.mzML.gz	Mzml
	AP_15052014_LTQ_S1_100pro_GlnA_pH5.mzML.gz	Mzml
	AP_15052014_LTQ_S1_100pro_GlnA_pH6.mzML.gz	Mzml

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Publications

Comparative transcriptome and proteome analysis reveals a global impact of the nitrogen regulators AreA and AreB on secondary metabolism in Fusarium fujikuroi.

Pfannmüller Andreas A Leufken Johannes J Studt Lena L Michielse Caroline B CB Sieber Christian M K CMK Güldener Ulrich U Hawat Susan S Hippler Michael M Fufezan Christian C Tudzynski Bettina B

PloS one 20170425 4

The biosynthesis of multiple secondary metabolites in the phytopathogenic ascomycete Fusarium fujikuroi is strongly affected by nitrogen availability. Here, we present the first genome-wide transcriptome and proteome analysis that compared the wild type and deletion mutants of the two major nitrogen regulators AreA and AreB. We show that AreB acts not simply as an antagonist of AreA counteracting the expression of AreA target genes as suggested based on the yeast model. Both GATA transcription f ...[more]

PMID: 28441411

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