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Quantitative phosphoproteomic analysis of mouse N2a cells

ABSTRACT: The aim of this project is to use integrated approach involving HPLC fractionation, IMAC affinity enrichment and mass spectrometry-based quantitative proteomics to quantify dynamic changes of protein phosphorylation in N2a cells towards different compounds.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

DISEASE(S): Neuroblastoma

SUBMITTER: Yongbo Wang

LAB HEAD: Yu Xue

PROVIDER: PXD004891 | Pride | 2018-10-26

REPOSITORIES: Pride

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			Action	DRS
	IgG.raw	Raw
	TbRI.raw	Raw
	txt_PTM20140816MS01_N2a_phospho_SILAC_3p....zip	Other

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Publications

Phosphoproteome-based kinase activity profiling reveals the critical role of MAP2K2 and PLK1 in neuronal autophagy.

Chen Lei-Lei LL Wang Yong-Bo YB Song Ju-Xian JX Deng Wan-Kun WK Lu Jia-Hong JH Ma Li-Li LL Yang Chuan-Bin CB Li Min M Xue Yu Y

Autophagy 20171004 11

Recent studies have demonstrated that dysregulation of macroautophagy/autophagy may play a central role in the pathogenesis of neurodegenerative disorders, and the induction of autophagy protects against the toxic insults of aggregate-prone proteins by enhancing their clearance. Thus, autophagy has become a promising therapeutic target against neurodegenerative diseases. In this study, quantitative phosphoproteomic profiling together with a computational analysis was performed to delineate the p ...[more]

PMID: 28933595

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