Proteomics

Dataset Information

0

Identification of autophosphorylationsites in recombinant Saccharomyces cerevisiae Ire1

ABSTRACT: This study reports autophosphorylation sites in the serine/threonine protein kinase Ire1. The cytosolic portion of Ire1 was expressed as an glutathione S transferase fusion protein in Escherichia coli. Phosphroyaltion sites in the recombinant wild type protein and a L745A mutant were mapped by mass spectrometry. Phosphorylation sites map of the activation loop in the protein kinase domain and the alphaEF-alphaF insertion loop. In the activation loop phosphorylation was observed at serine 837, serine 840, serine 844, and threonine 844. Phosphorylaton was also observed at serine 850 in the P+1 loop. In the alphaEF-alphaF insertion loop phosphorylation was observed at threonine 873, serine 877, serine 878, threonine 881, serine 884, serine 885, and serine 887.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Martin Schroeder  

LAB HEAD: Martin Schröder

PROVIDER: PXD004924 | Pride | 2017-06-09

REPOSITORIES: pride

Json Xml

Dataset's files

Source:
Action DRS
100526_07_PR10_0021_L745A.RAW Raw
100527_05_PR10_0021_WT.RAW Raw
GPM28700000059.xml Xml
GPM28700000061.xml Xml
Items per page:
1 - 4 of 4
altmetric image

Publications

Bypass of Activation Loop Phosphorylation by Aspartate 836 in Activation of the Endoribonuclease Activity of Ire1.

Armstrong Michael C MC   Šestak Sergej S   Ali Ahmed A AA   Sagini Hanan A M HAM   Brown Max M   Baty Karen K   Treumann Achim A   Schröder Martin M  

Molecular and cellular biology 20170728 16

The bifunctional protein kinase-endoribonuclease Ire1 initiates splicing of the mRNA for the transcription factor Hac1 when unfolded proteins accumulate in the endoplasmic reticulum. Activation of <i>Saccharomyces cerevisiae</i> Ire1 coincides with autophosphorylation of its activation loop at S840, S841, T844, and S850. Mass spectrometric analysis of Ire1 expressed in <i>Escherichia coli</i> identified S837 as another potential phosphorylation site <i>in vivo</i> Mutation of all five potential  ...[more]

Similar Datasets

Genomics
| PRJNA951886 | ENA
Transcriptomics Transcription profiling of human NIH 3T3 inducible cell line to study the role of PKR in regulating gene expression
2004-01-30 | E-MEXP-50 | biostudies-arrayexpress
Proteomics A novel blood brain barrier-permeable IRE1 inhibitor prevents Glioblastoma relapse in mice
2023-07-20 | PXD026908 | Pride
Proteomics Identification of cell cycle dependent interaction partners of the septins by quantitative mass spectrometry
2016-01-28 | PXD002561 | Pride
Clinical A Study Evaluating the Combination of Encorafenib and Cetuximab Versus Irinotecan/Cetuximab or Infusional 5-fluorouracil (5-FU)/Folinic Acid (FA)/Irinotecan (FOLFIRI)/Cetuximab in Chinese Patients With BRAF V600E Mutant Metastatic Colorectal Cancer.
| 2386568 | ecrin-mdr-crc
Proteomics BS3 crosslinking of GRC3 and Las1
2019-09-11 | PXD014738 | Pride
Proteomics Stable isotope dynamic labelling of secretomes: Strataclean Linearity C4PR_LIV
2019-10-08 | PXD009838 | Pride
Transcriptomics Transcription profiling of mouse NIH3T3 cells over-expressing RNA-dependent serine/threonine protein kinase (PKR)PKR
2008-06-15 | E-GEOD-682 | biostudies-arrayexpress
Transcriptomics RNA sequencing identifies novel regulated IRE1-dependent decay targets that affect multiple myeloma survival and proliferation
2022-04-06 | GSE152070 | GEO
Transcriptomics Trout_Muscle_diet&selection
2010-05-15 | E-GEOD-16577 | biostudies-arrayexpress