Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome of Young and Old Murine Diaphragm and Associaated Changes in Contractile Properties

ABSTRACT: Proteomic analysis of young and old murine diaphragm and associated changed in contractile aproperties. Redox sensitive Cysteine residues from both adult and old diaphragm tissues examined.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Diaphragm

SUBMITTER: Brian McDonagh

LAB HEAD: Brian McDonagh

PROVIDER: PXD005014 | Pride | 2018-02-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	s1.mgf	Mgf
	s1.msf	Msf
	s1.raw	Raw
	s10.mgf	Mgf
	s10.msf	Msf

Items per page:

1 - 5 of 36

Publications

Advanced aging causes diaphragm functional abnormalities, global proteome remodeling, and loss of mitochondrial cysteine redox flexibility in mice.

Kelley Rachel C RC McDonagh Brian B Ferreira Leonardo F LF

Experimental gerontology 20171228

<h4>Aim</h4>Inspiratory muscle (diaphragm) function declines with age, contributing to exercise intolerance and impaired airway clearance. Studies of diaphragm dysfunction in rodents have focused on moderate aging (~24months); thus, the impact of advanced age on the diaphragm and potential mechanisms of dysfunction are less clear. Therefore, we aimed to define the effects of advanced age on the mechanics, morphology, and global and redox proteome of the diaphragm.<h4>Methods</h4>We studied diaph ...[more]

PMID: 29289553

Similar Datasets

Proteomic Analysis of Diaphragm after Chronic Heart Failure_Males

Project description:Proteomic analysis of diaphragm tissue from rats subjected to chronic heart failure. Redox sensitive Cysteine residues from both adult and old diaphragm tissues examined

2020-04-15 | PXD012500 | Pride

miR-181a Restores Mitochondrial Dynamics in Skeletal Muscle Ageing

Project description:Global label free analysis of quadricesp skeletal muscle tissue from adult and old mice subjected to 15 min of isometric contractions.

2020-04-15 | PXD010091 | Pride

Defective Mitochondrial Lipid Oxidation Results in the Pathological Accumulation of Fat After Rotator Cuff Tear

Project description:Myosteatosis, also known as fatty infiltration, is the pathological accumulation of lipid that occurs in conjunction with atrophy and fibrosis following rotator cuff injury. Little is known about the mechanisms by which lipid accumulates in myosteatosis, but many studies have demonstrated the degree of lipid infiltration negatively correlates with muscle function and regeneration. Identifying how reduced mechanical loading activates molecular pathways that lead to myosteatosis could help to develop targeted therapies to improve functional outcomes after rotator cuff repair. Our objective was to use cutting compare muscle fiber contracility, proteomic, RNA sequencing and shotgun metabolomics along with bioinformatics to identify potential pathways and cellular processes that are dysregulated after rotator cuff teaar.

2020-04-15 | PXD009034 | Pride

Proteomics on mdxmTR Mice

Project description:Label free proteomics of skeletal muscle from mdxmTR mice.

2022-03-01 | PXD010507 | Pride

Laminin-deficient muscular dystrophy, dy/dy diaphragm

Project description:Laminin (merosin) deficient muscular dystrophy in dy/dy mouse diaphragm muscle, 8 weeks old Keywords: muscle, muscular dystrophy, laminin, merosin, diaphragm, mouse

2006-03-16 | GSE3252 | GEO

Transcription profiling of rat treated with streptozotocin or buffer alone at age of 8 weeks, diaphragm muscle

Project description:8 week old rats injected with streptozotocin or buffer alone at age of 8 weeks, diaphragm muscle obtained at age of 12 weeks (thus animals were diabetic for 4 weeks) Experiment Overall Design: Three control rats (normal diaphragm #1, 2, 3) Experiment Overall Design: Three diabetic rats (diabetic diaphragm #1, 2, 3)

2009-11-28 | E-GEOD-4653 | biostudies-arrayexpress

Diaphragm in Diabetes

2009-11-13 | GSE4653 | GEO

Effect of individualized PEEP guided by driving pressure on diaphragm function in patients undergoing laparoscopic radical resection of colorectal cancer

Project description:Interventions: R:PEEP=0;F:PEEP=5;P:Individualized PEEP Primary outcome(s): Diaphragm mobility;End expiratory diaphragm thickness;Diaphragm thickness at the end of inspiration;Change rate of diaphragm thickness;Postoperative diaphragmatic dysfunction Study Design: Parallel

| 84106 | ecrin-mdr-crc

Dystrophic single myofibers vs control single myofibers

Project description:MicroRNA-expression profile of dystrophic single fibres vs wild type single fibers isolated from different muscle of mdx and c57bl mice. Myofibers were isolated from different muscle type (tibialis, diaphragm and quadriceps of gender- (male) and age- (3 month old and half) matched wt and dystrophic mice).

2012-12-21 | GSE21219 | GEO

Zucker Diabetic Fatty (ZDF) Rat Study - Diaphragm vs. Sternohyoid

Project description:Type 2 diabetes differs from type 1 diabetes in its pathogenesis. Type 1 diabetic diaphragm has altered gene expression which includes lipid and carbohydrate metabolism, ubiquitination and oxidoreductase activity. The objectives of the present study were to assess respiratory muscle gene expression changes in type 2 diabetes and to determine whether they are greater for the diaphragm than an upper airway muscle. Diaphragm and sternohyoid muscle from Zucker diabetic fatty (ZDF) rats were analyzed with Affymetrix gene expression arrays. The two muscles had 97 and 102 genes, respectively, with at least ±1.5-fold significantly changed expression with diabetes, and these were assigned to gene ontology groups based on over-representation analysis. Several significantly changed groups were common to both muscles, including lipid metabolism, carbohydrate metabolism, muscle contraction, ion transport and collagen, although the number of genes and the specific genes involved differed considerably for the two muscles. In both muscles there was a shift in metabolism gene expression from carbohydrate metabolism toward lipid metabolism, but the shift was greater and involved more genes in diabetic diaphragm than diabetic sternohyoid muscle. Groups present in only diaphragm were blood circulation and oxidoreductase activity. Groups present in only sternohyoid were immune & inflammation and response to stress & wounding, with complement genes being a prominent component. In conclusion, type 2 diabetes-induced gene expression changes in respiratory muscles has both similarities and differences relative to previous data on type 1 diabetes gene expression. Furthermore, the diabetic alterations in gene expression differ between diaphragm and sternohyoid. 18-week-old Zucker diabetic fatty rats: 6 normal and 5 diabetic diaphragms and sternohyoids.

2014-05-12 | E-GEOD-21791 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data