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Identification of proteins in a Salmonella SPI-1 type III secretion injectisome basal body mutant

ABSTRACT: We determined the cryo-EM structure of a secretion incompetent PrgH130-392 type III secretion injectisome basal body mutant at 6.3 Å. The map shows no density attributable to the inner rod or needle and the periplasmic gate in a closed conformation. We used LC-MS/MS to idensitfy the constituent proteins showing the predominant presence of the major inner- and outer basal body proteins (PrgH+PrgK and InvG respectively) and export apparatus protein SpaP.

INSTRUMENT(S): impact II

ORGANISM(S): Salmonella Typhimurium (strain Lt2 / Sgsc1412 / Atcc 700720)

SUBMITTER: Liam Worrall

LAB HEAD: Natalie Strynadka

PROVIDER: PXD005133 | Pride | 2016-12-19

REPOSITORIES: Pride

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Publications

Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body.

Worrall L J LJ Hong C C Vuckovic M M Deng W W Bergeron J R C JRC Majewski D D DD Huang R K RK Spreter T T Finlay B B BB Yu Z Z Strynadka N C J NCJ

Nature 20161214 7634

The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. This syringe-shaped 3.5-MDa macromolecular assembly spans both bacterial membranes and that of the infected host cell. The internal channel formed by the injectisome allows for the direct delivery of partially unfolded virul ...[more]

PMID: 27974800

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