Dataset Information

Inefficient secretion of anti-sigma factor FlgM inhibits bacterial motility at high temperature

ABSTRACT: Regulation of gene expression according to the environmental conditions is vitally important for all living organisms. Temperature is one of the key cues for microorganisms that can exist in different environments, and several mechanisms of temperature-dependent re-allocation of cellular resources have been described. Here we compared transcriptome and proteome of E. coli grown at basal (37°C) and febrile temperatures (42°C) of the mammalian host. We demonstrated that the downregulation of Escherichia coli motility at high temperature primarily occurred due to the malfunction of secretion apparatus, apparently related to the disassembly of the flagellar basal body. For proteome analysis we performed data-independent acquisition (DIA) mass spectrometry via Spectronaut. The Spectronaut file can be assessed with the freely available Spectronaut viewer (https://www.biognosys.com/technology/spectronaut-viewer).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Timo Glatter

LAB HEAD: Victor Sourjik

PROVIDER: PXD012611 | Pride | 2019-08-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180308_120532_Temp3-IaroslavRudenko_SpectronautAnalysis.sne	Other
	EcoliK12_fwd.fasta	Fasta
	ExperimentalSetUp.csv	Csv
	Temp-3-1.raw	Raw
	Temp-3-10.raw	Raw

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Publications

Inefficient Secretion of Anti-sigma Factor FlgM Inhibits Bacterial Motility at High Temperature.

Rudenko Iaroslav I Ni Bin B Glatter Timo T Sourjik Victor V

iScience 20190520

Temperature is one of the key cues that enable microorganisms to adjust their physiology in response to environmental changes. Here we show that motility is the major cellular function of Escherichia coli that is differentially regulated between growth at normal host temperature of 37°C and the febrile temperature of 42°C. Expression of both class II and class III flagellar genes is reduced at 42°C because of lowered level of the upstream activator FlhD. Class III genes are additionally represse ...[more]

PMID: 31170626

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