Proteomics

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Podocyte degradomics - N-Degradomic Analysis Reveals a Proteolytic Network Processing the Podocyte Cytoskeleton

ABSTRACT: Regulated intracellular proteolysis is essential in maintaining the integrity of podocytes and the glomerular filtration barrier of the kidney. Altered proteolytic substrate turnover has been associated with various glomerular diseases ranging from diabetic nephropathy to focal and segmental glomerulosclerosis. However, thus far it has not been possible to systematically identify proteolytically cleaved proteins although some of the proteases have been characterized. Here we applied TAILS to map N-termini in the mouse glomeruli proteome and to determine and quantify N termini in podocyte cell cultures challenged with PAN.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Pitter Huesgen  

LAB HEAD: Pitter F. Huesgen

PROVIDER: PXD005139 | Pride | 2017-07-20

REPOSITORIES: Pride

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Action DRS
20150806_UC_Glomeruli_150615_Q1_01.raw Raw
20151214_UC_0089_151202MaRi_03.raw Raw
2016-04_MQ_glomeruli_search.zip Other
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Publications

Regulated intracellular proteostasis, controlled in part by proteolysis, is essential in maintaining the integrity of podocytes and the glomerular filtration barrier of the kidney. We applied a novel proteomics technology that enables proteome-wide identification, mapping, and quantification of protein N-termini to comprehensively characterize cleaved podocyte proteins in the glomerulus <i>in vivo</i> We found evidence that defined proteolytic cleavage results in various proteoforms of important  ...[more]

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