STUDY OF THE PLASMA MEMBRANE PROTEOME DYNAMICS REVEALS NOVEL TARGETS OF THE NITROGEN REGULATION IN YEAST

ABSTRACT: Yeast cells, to be able to grow on a wide variety of nitrogen sources, regulate the set of nitrogen transporters present at their plasma membrane. Such regulation relies on both transcriptional and post-translational events. While large-scale microarray studies have identified most nitrogen-sensitive genes, nitrogen-induced post-translational regulation has only been studied for very few proteins among which the general amino acid permease Gap1. Adding a preferred nitrogen source to proline-grown cells triggers Gap1 endocytosis and vacuolar degradation in an Rsp5-Bul1/2-dependent manner. Here, we used a large-scale proteomic approach to follow the dynamics of the plasma membrane proteome after addition of a preferred nitrogen source. We identified new targets of the nitrogen regulation and four transporters of poor nitrogen sources—Put4, Opt2, Dal5 and Ptr2—that rapidly decrease in abundance. Although the kinetics is different for each transporter, we found that three of them—Put4, Dal5 and Ptr2— are endocytosed, like Gap1, in an Rsp5-dependent manner and degraded in the vacuole. Finally, we showed that Gap1 stabilization at the plasma membrane, through deletion of Bul proteins, regulates the abundance of Put4, Dal5 and Ptr2.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Suspension Culture, Haploid

SUBMITTER: Hervé Degand  

LAB HEAD: Pierre Morsomme

PROVIDER: PXD005273 | Pride | 2017-07-10

REPOSITORIES: Pride