N-Linked Glycoprotein Profiles of Human Breast Normal/Benign Cell Lines

ABSTRACT: Secreted proteins and transmembrane proteins with extracellular domains are frequently glycosylated; this group of proteins includes those that participate in the various intercellular junctions and signaling pathways of an epithelium. In this study we characterized the differences in glycoprotein expression between claudin-low and other breast cell lines using a dataset of 26 breast cell lines in which the glycoproteins were identified and quantitated by liquid chromatography/ tandem mass spectrometry. Our goals are to characterize the glycoproteome of a set of claudin-low lines, compare them to basal, luminal and non-malignant cells and to identify drugs that may be especially effective on these cell lines. These five non-malignant breast cells (3 normal from 3 donors: HMEpC-p3, HMEC-p10 and HMEC#3-P11; 2 benign: MCF10A and MCF12A) data are a part of 26 breast cell lines we analyzed.

INSTRUMENT(S): LTQ, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Ten-Yang Yen  

LAB HEAD: Ten-Yang Yen

PROVIDER: PXD005339 | Pride | 2017-03-23

REPOSITORIES: Pride