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TGFBR2-dependent Alterations of Exosomal Cargo in DNA Mismatch Repair-deficient HCT116 Colorectal Cancer Cells

ABSTRACT: DNA mismatch repair-deficient colorectal tumors (CRC) frequently show coding microsatellite frameshift mutations in the MSI tumor driver Transforming Growth Factor Beta Receptor Type 2 (TGFBR2) leading to cellular alteration of MSI tumor cells [Lee, J., Warnken, U., Schnölzer, M., Gebert, J. & Kopitz, J. (2015) Protein Sci. 24, 1686-94; Lee, J., Ballikaya, S., Schonig, K., Ball, C. R., Glimm, H., Kopitz, J. & Gebert, J. (2013) PloS one. 8, e57074]. Whether this receptor expression in MSI tumor cells also impacts the exosomal proteomic cargo of these cells is still unknown.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Fabia Fricke

LAB HEAD: Prof. Juergen Kopitz

PROVIDER: PXD005620 | Pride | 2017-04-07

REPOSITORIES: Pride

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	150116_JK2358_1.RAW	Raw
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TGFBR2-dependent alterations of exosomal cargo and functions in DNA mismatch repair-deficient HCT116 colorectal cancer cells.

Fricke Fabia F Lee Jennifer J Michalak Malwina M Warnken Uwe U Hausser Ingrid I Suarez-Carmona Meggy M Halama Niels N Schnölzer Martina M Kopitz Jürgen J Gebert Johannes J

Cell communication and signaling : CCS 20170404 1

<h4>Background</h4>Colorectal cancers (CRCs) that lack DNA mismatch repair function exhibit the microsatellite unstable (MSI) phenotype and are characterized by the accumulation of frameshift mutations at short repetitive DNA sequences (microsatellites). These tumors recurrently show inactivating frameshift mutations in the tumor suppressor Transforming Growth Factor Beta Receptor Type 2 (TGFBR2) thereby abrogating downstream signaling. How altered TGFBR2 signaling affects exosome-mediated commu ...[more]

PMID: 28376875

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