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Phosphoproteomics of STMN1 knockdown cells

ABSTRACT: Phosphopeptides were enriched from STMN1 knockdown cells via the TiO2 HAMMOC approach, followed by SWATH-MS data acquisition for comparative proteomics analysis.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Hwee Tong Tan

LAB HEAD: Maxey Chung Ching Ming

PROVIDER: PXD005865 | Pride | 2018-02-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150514_HSC-HKD-ESC-EKD_IDA_1.wiff	Wiff
	150514_HSC-HKD-ESC-EKD_IDA_2.wiff	Wiff
	150514_HSC-HKD-ESC-EKD_IDA_3.wiff	Wiff
	150514_HSC-HKD-ESC-EKD_IDA_all.group	Other
	PhosphoproteomicsSTMN1_IDA.mgf	Mgf

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Publications

Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.

Tan Hwee Tong HT Chung Maxey Ching Ming MCM

Proteomics 20180330 8

In this communication, we present the phosphoproteome changes in an isogenic pair of colorectal cancer cell lines, viz., the poorly metastatic HCT-116 and the highly metastatic derivative E1, upon stathmin-1 (STMN1) knockdown. The aim was to better understand how the alterations of the phosphoproteins in these cells are involved in cancer metastasis. After the phosphopeptides were enriched using the TiO<sub>2</sub> HAMMOC approach, comparative proteomics analysis was carried out using sequential ...[more]

PMID: 29460479

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