Dataset Information

Systemic perturbation of keratinocyte homeostasis

ABSTRACT: We performed comprehensive proteome profiling (ECM and cell lysate) of human collagen VII deficient and control epidermal keratinocytes to generate global and detailed images of dysregulated epidermal molecular pathways linked to loss of collagen VII. These revealed downregulation of interaction partners of collagen VII, but also increased abundance of S100 pro-inflammatory proteins and enhanced activity of lysosomal proteases. Thus, loss of a single extracellular structural protein, collagen VII, induced persistent fibrosis enabling inflammatory and tissue destabilizing activities of keratinocytes.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Keratinocyte

DISEASE(S): Recessive Dystrophic Epidermolysis Bullosa,Disease Free

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD005873 | Pride | 2018-01-16

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20140806_KT_KerProt_ECM_1_60.raw	Raw
	20140806_KT_KerProt_ECM_1_70.raw	Raw
	20140806_KT_KerProt_ECM_1_70_140814160453.raw	Raw
	20140806_KT_KerProt_ECM_1_75.raw	Raw
	20140806_KT_KerProt_ECM_1_80.raw	Raw

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Publications

Combinatorial Omics Analysis Reveals Perturbed Lysosomal Homeostasis in Collagen VII-deficient Keratinocytes.

Thriene Kerstin K Grüning Björn Andreas BA Bornert Olivier O Erxleben Anika A Leppert Juna J Athanasiou Ioannis I Weber Ekkehard E Kiritsi Dimitra D Nyström Alexander A Reinheckel Thomas T Backofen Rolf R Has Cristina C Bruckner-Tuderman Leena L Dengjel Jörn J

Molecular & cellular proteomics : MCP 20180111 4

The extracellular matrix protein collagen VII is part of the microenvironment of stratified epithelia and critical in organismal homeostasis. Mutations in the encoding gene <i>COL7A1</i> lead to the skin disorder dystrophic epidermolysis bullosa (DEB), are linked to skin fragility and progressive inflammation-driven fibrosis that facilitates aggressive skin cancer. So far, these changes have been linked to mesenchymal alterations, the epithelial consequences of collagen VII loss remaining under- ...[more]

PMID: 29326176

Publication: 1/2

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