Proteomics

Dataset Information

0

Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation


ABSTRACT: The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel NK cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1,300 cell surface and ~7,200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human) Human Cytomegalovirus (hhv-5) (human Herpesvirus 5)

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Cytomegalovirus Retinitis

SUBMITTER: Yagnesh Umrania  

LAB HEAD: Michael Weekes

PROVIDER: PXD005883 | Pride | 2017-02-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CMV_US12_21_PM_A_B_C_WCL_A_B_C_TTT_LTLTLT_TrypsP_SN100_IS0_5_IIW_0_5.txt Txt
Series_1_PM_SCX10.raw Raw
Series_1_PM_SCX150.raw Raw
Series_1_PM_SCX25.raw Raw
Series_1_PM_SCX40.raw Raw
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Publications


The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel natural killer (NK) cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1300 cell surface and ~7200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecu  ...[more]

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