Dataset Information

The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella.

ABSTRACT: Galleria mellonella is a well-established model species regularly employed in the study of the insect immune response at cellular and humoral levels to investigate fungal pathogenesis and biocontrol agents. A cellular and proteomic analysis of the effect of culture filtrate of three EPF species on the immune system of G. mellonella was performed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Galleria Mellonella

TISSUE(S): Blood

SUBMITTER: James Carolan

LAB HEAD: Jim Carolan

PROVIDER: PXD005939 | Pride | 2017-05-29

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 17

Publications

The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella.

Mc Namara Louise L Carolan James C JC Griffin Christine T CT Fitzpatrick David D Kavanagh Kevin K

Journal of insect physiology 20170522

Galleria mellonella is a well-established model species regularly employed in the study of the insect immune response at cellular and humoral levels to investigate fungal pathogenesis and biocontrol agents. A cellular and proteomic analysis of the effect of culture filtrate of three entomopathogenic fungi (EPF) species on the immune system of G. mellonella was performed. Treatment with Beauveria caledonica and Metarhizium anisopliae 96h culture filtrate facilitated a significantly increased yeas ...[more]

PMID: 28545993

Similar Datasets

Project description:A genomic insight into how an insect pest responds to the infection of a fungal insect pathogen, such as Beauveria bassiana, is critical for alternative strategy of insect pest contol based on fungal insecticides but has not been well probed. Here we constructed three pairs of digital expression libraries (transcriptomes) of Plutella xylostella (global lepidopteran pest) larvae 24, 36 and 48 hours post treatment of infection (hptI) and control (hptC) to reveal the host response to B. bassiana infection at genomic level. The paired libraries comprised 2144, 3200 and 2967 differentially expressed genes (DEGs) of P. xylostella at 24, 36 and 48 hptI/hptC, respectively. These DEGs were enriched in various immune pathways activated by the fungal infection, such as the pathways of complement and coagulation cascades, protein digestion and absorption, and drug metabolism - cytochrome P450. We found that 24 hptI was critical either for the cuticular penetration of B. bassiana or for the initial activation of the host defense system. The host immune response peaked at 36 hptI so that multiple defense mechanisms were activated against the fungal entry into the host hemocoel. At 48 hptI, many host genes involved in immunity and metabolism were downregulated, suggesting a success of fungal localization in the host hemocoel by overcoming the host defense reaction. Finally, we revealed that several fungal pathways could play important roles in the host-pathogen interaction, such as antioxidant activity, peroxidase activity and proteolysis. Up to 1636 fungal genes were co-expressed at the three time points, and 116 of them encode putative secretion proteins. Our results provide a novel insight into the pathogen-insect interaction and help to probe molecular mechanisms involved in the control of P. xylostella by B. bassiana. Here we constructed three pairs of digital expression libraries (transcriptomes) of Plutella xylostella (global lepidopteran pest) larvae 24, 36 and 48 hours post treatment of infection (hptI) and control (hptC) to reveal the host response to B. bassiana infection at genomic level

			Action	DRS
	BC1.raw	Raw
	BC2.raw	Raw
	BC3.raw	Raw
	BC4.raw	Raw
	MaxQuantOutput.tar.gz	Other

Dataset Information

The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella.

Dataset's files

Publications

The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets