Dataset Information

Toxicoproteomic and lipidomic analysis of human lung cells chronically exposed to MWCNT

ABSTRACT: Multi-walled carbon nanotubes (MWCNT) present a wide variety of exciting application opportunities. As MWCNT are produced in large quantities, occupational exposure and human health is of particular concern. However, there is no consensus regarding their potential harmful effects. In particular, chronic exposure to MWCNT and mechanisms of their action at protein and lipid levels are unknown. In this study, we aimed to investigate effects of long-term chronic exposure to MWCNT on cellular proteome and lipidome. Since the lung is the major target organ, an in vitro normal bronchial epithelial cell model was used. To better mimic exposure at occupational settings, cells were chronically exposed for 13 weeks to low-doses of MWCNT. MWCNT-treatment increased ROS levels in cells without increasing DNA damage and resulted in differential expression of multiple apoptotic proteins. A shotgun proteomic and lipidomic analysis of the MWCNT-exposed cells showed that of amongst the >5000 identified protein s,groups; more than 200 were altered in treated cells. Functional analysis revealed association of these differentially regulated proteins in various cellular processes such as cell death and survival, cellular assembly and organization. Similarly, the lipid profile of the MWCNT treated cells showed accumulation of multiple lipid classes. This is first study to present results indicating that long-term MWCNT-exposure of human normal lung cells at occupationally relevant low-dose may alter both the proteome and the lipidome profile of target epithelial cells in the lung.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

SUBMITTER: Animesh Sharma

LAB HEAD: Geir Slupphaug

PROVIDER: PXD005970 | Pride | 2020-05-06

REPOSITORIES: Pride

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Characterization of the proteome and lipidome profiles of human lung cells after low dose and chronic exposure to multiwalled carbon nanotubes.

Phuyal Santosh S Kasem Mayes M Knittelfelder Oskar O Sharma Animesh A Fonseca Davi de Miranda DM Vebraite Vaineta V Shaposhnikov Sergey S Slupphaug Geir G Skaug Vidar V Zienolddiny Shanbeh S

Nanotoxicology 20180119 2

The effects of long-term chronic exposure of human lung cells to multi-walled carbon nanotubes (MWCNT) and their impact upon cellular proteins and lipids were investigated. Since the lung is the major target organ, an in vitro normal bronchial epithelial cell line model was used. Additionally, to better mimic exposure to manufactured nanomaterials at occupational settings, cells were continuously exposed to two non-toxic and low doses of a MWCNT for 13-weeks. MWCNT-treatment increased ROS levels ...[more]

PMID: 29350075

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