Proteomics

Dataset Information

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Large scale investigation of the phage Vp16 PDF activity

ABSTRACT: Prokaryotic proteins must be deformylated before the removal of their first methionine. Peptide deformylase (PDF) is indispensable and guarantees this cotranslational mechanism. Recent genome sequencing and annotation studies highlighted over 2x104 putative peptide deformylase sequences. Furthermore, unpredicted modified bacterial PDF genes have been retrieved from many phages. Sequence comparisons with other known PDFs reveal that viral PDFs are devoid of the key ribosome-interacting C-terminal region. Little is known regarding these viral PDFs, including the capacity of the corresponding encoded proteins to ensure deformylase activity in vitro or in vivo. This investigation determines the activity of the recombinant Vp16 PDF in E. coli PDF defective cell to remove the N-formyl group at the protein N-terminus.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli

SUBMITTER: Willy Bienvenut  

LAB HEAD: Willy Vincent Bienvenut

PROVIDER: PXD006008 | Pride | 2017-09-25

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20120814_WIB_VB-2h_SCX_frac_2-5_CID_Ch1.dat Other
20120814_WIB_VB-2h_SCX_frac_2-5_CID_Ch1.raw Raw
20120814_WIB_VB-2h_SCX_frac_2-5_CID_Ch1.raw.mgf Mgf
20120814_WIB_VB-2h_SCX_frac_2-5_CID_Ch2.dat Other
20120814_WIB_VB-2h_SCX_frac_2-5_CID_Ch2.raw Raw
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Publications

The C-terminal residue of phage Vp16 PDF, the smallest peptide deformylase, acts as an offset element locking the active conformation.

Grzela Renata R   Nusbaum Julien J   Fieulaine Sonia S   Lavecchia Francesco F   Bienvenut Willy V WV   Dian Cyril C   Meinnel Thierry T   Giglione Carmela C  

Scientific reports 20170908 1

Prokaryotic proteins must be deformylated before the removal of their first methionine. Peptide deformylase (PDF) is indispensable and guarantees this mechanism. Recent metagenomics studies revealed new idiosyncratic PDF forms as the most abundant family of viral sequences. Little is known regarding these viral PDFs, including the capacity of the corresponding encoded proteins to ensure deformylase activity. We provide here the first evidence that viral PDFs, including the shortest PDF identifie  ...[more]

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