Proteomics

Dataset Information

0

System-wide analyses reveal essential roles of N-terminal protein modification in bacterial physiology


ABSTRACT: Removal of the N-terminal formyl group on nascent proteins by peptide deformylase (PDF) is the most prevalent protein modification in bacteria that impacts over 90% of the proteome. PDF is essential and a critical target of antibiotic development; however, its role in bacterial physiology remains a long-standing question. In this work, we used system-wide and time-resolved analyses of the E. coli translatome and proteome to investigate the consequences of PDF inhibition at different stages. We found that PDF inhibition results in the rapid activation of cellular stress responses, especially those associated with defects in protein folding and perturbations at the bacterial inner membrane, followed by a global downregulation of metabolic pathways. Functional assays reveal the rapid hyperpolarization of the plasma membrane and impaired membrane integrity upon PDF inhibition, suggesting that disruption of plasma membrane is the most immediate and primary consequence of formyl group retention on nascent proteins. Our work resolves the physiological function of a ubiquitous N-terminal protein modification and uncovers its crucial role in maintaining the proper structure and function of the bacterial membrane.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Escherichia Coli

SUBMITTER: Jeff Jones  

LAB HEAD: Tsui-Fen Chou

PROVIDER: PXD032725 | Pride | 2022-07-20

REPOSITORIES: Pride

Similar Datasets

2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
2023-12-08 | PXD037670 | Pride
2021-03-10 | PXD017286 | Pride
2020-11-24 | PXD011771 | Pride
2019-10-23 | PXD013162 | Pride
2022-08-20 | PXD030174 | Pride
2015-07-21 | PXD002012 | Pride
2015-07-22 | PXD001983 | Pride
2015-07-21 | PXD001979 | Pride
2020-01-07 | PXD016521 | Pride