Dataset Information

Proteomics identification of regulated proteins during cardyomyocyte differentiation

ABSTRACT: The rat cardio-myoblast cell line H9C2 has emerged as an important tool for studying cardiac development and toxicology. We present here a rigorous proteomic analysis that for the first time studied changes of proteins during H9C2 differentiation into cardiomyocyte-like cells over time. Quantitative mass spectrometry followed by gene ontology (GO) enrichment analysis revealed early changes in protein pathways that enable cardiac muscle morphogenesis/contraction and suggested an involvement of proteins relevant to sphingolipid synthesis. This early differentiation was followed by differentiation-induced alterations in cation transport and beta-oxidation at later time points. Applying a two-way ANOVA to study the temporal profile of H9C2 differentiation in further detail revealed eight clusters of co-regulated proteins that could be associated to early, late, continuous and transient up- and downregulation. Subsequent Reactome pathway analysis based on these eight clusters further corroborated and detailed the results of the GO analysis. Specifically, this analysis confirmed proteins related to pathways in muscle contraction as early and transiently upregulated, and proteins relevant to ECM matrix organization as early downregulated, while changes related to cardiac metabolism occurred at later time points. Our results are in line with a ‘function follows form’ model of differentiation, whereby early and transient changes of cellular morphology enable subsequent changes that are relevant for the characteristic physiology of cardiac cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): H9c2 Cell, Cardiocyte Differentiation

SUBMITTER: Impens Francis

LAB HEAD: Heinrich Huber

PROVIDER: PXD006115 | Pride | 2018-11-07

REPOSITORIES: Pride

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			Action	DRS
	H01538_6092_EXT435_HuberLab_KylieClarke_Shotgun_1.raw	Raw
	H01540_6092_EXT435_HuberLab_KylieClarke_Shotgun_2.raw	Raw
	H01542_6092_EXT435_HuberLab_KylieClarke_Shotgun_3.raw	Raw
	H01544_6092_EXT435_HuberLab_KylieClarke_Shotgun_C1.raw	Raw
	H01546_6092_EXT435_HuberLab_KylieClarke_Shotgun_C2.raw	Raw

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Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation.

Kankeu Cynthia C Clarke Kylie K Van Haver Delphi D Gevaert Kris K Impens Francis F Dittrich Anna A Roderick H Llewelyn HL Passante Egle E Huber Heinrich J HJ

Molecular omics 20180601 3

The rat cardiomyoblast cell line H9C2 has emerged as a valuable tool for studying cardiac development, mechanisms of disease and toxicology. We present here a rigorous proteomic analysis that monitored the changes in protein expression during differentiation of H9C2 cells into cardiomyocyte-like cells over time. Quantitative mass spectrometry followed by gene ontology (GO) enrichment analysis revealed that early changes in H9C2 differentiation are related to protein pathways of cardiac muscle mo ...[more]

PMID: 29770421

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